神经前体细胞的增殖和分化及其对Parkinson病的治疗作用  

THE PROLIFERATION AND DIFFERENTIATION OF NEURAL PROGENITOR CELLS AND THEIR REGULATION ON PARKINSON'S DISEASE

作　　者：孙朝晖[1] 赖燕来[1] 李鹏[1] 张丽丽[1] 左焕琮[1] 谢佐平[1] 

机构地区：[1]清华大学生物科学与技术系,生物膜与膜生物工程国家重点实验室,北京100084

出　　处：《神经解剖学杂志》2003年第4期359-364,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金 ( No. 30 0 70 2 45 )资助项目

摘　　要：本实验分离 1 1～ 1 5 d胎鼠的大脑皮层 ( CP)和中脑的神经前体细胞 ( MP) ,观察它们在体外的增殖和分化及其对帕金森病 ( PD)的治疗作用。结果发现 ,CP较 MP生长迅速 ,容易形成神经球 ,加入 N2 / B2 7有助于神经球的形成 ;有 /无血清分化后 ,CP和 MP都能发育成微管相关蛋白 -2 ( MAP-2 )阳性细胞 ,其中 MP倾向于发育成酪氨酸羟化酶 ( TH)阳性细胞。将 MP或 CP植入6 -OHDA损伤的大鼠纹状体后 ,MP发育成的 TH+神经元数量明显地较 CP移植组和对照组者多 ( P<0 .0 5 )且 MP移植后 4～ 8周旋转行为发生明显改善 ,与 CP移植组和对照组相比有显著性差异 ( P<0 .0 5 )。上述结果提示 ,虽然 CP在体外的增殖能力较MP强 ,但 MP更易于分化成 TH阳性神经元并改善 PD大鼠的旋转行为 ,此点对于神经前体细胞治疗 PD的应用极其重要。Cortical (CP) and mesencephalic progenitor cells (MP) from E11～15 embryonic rats were proliferated and differentiated in vitro and then they were transplanted into the striatum of the 6-OHDA-lesioned rats to observe the therapeutic effects on Parkinson's disease (PD). It had been found that CP proliferated faster than MP, which formed much more neurospheres than MP after they were cultured in the serum-free medium. Their ability to form neurospheres was augmented after N 2/B 27 were added. After being differentiated in the serum/serum-free medium in vitro, both CP and MP could develop into microtubule associated protein-positive (MAP-2 +) cells, but MP was inclined to differentiate into tyrosine hydroxylase-positive (TH +) cells. After CP or MP was transplanted into the striatum of the 6-OHDA-lesioned rat, more TH + neurons were found in MP group than that in control or CP group, which also showed significant improvement of the rotational behavior. These results suggest that CP had stronger ability to proliferate in vitro, but MP was inclined to differentiate into TH + cells and the rotational behavior in PD rats was attenuated, which is very important for the clinical therapy of PD using neural progenitor cells.

关 键 词：神经前体细胞 增殖 分化 PARKINSON病 治疗作用 帕金森病 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R742.5[医药卫生—基础医学]