莱茵衣藻叶绿体atpA启动子在杜氏盐藻中转录活性的检测  

Transcription activities of the atpA promoter of Chlamydomonas reinhartii chloroplast in Dunaliella salina

作　　者：潘卫东[1] 吕玉民[1] 张贵星[1] 牛向丽[1] 侯桂琴[1] 薛乐勋[1] 

机构地区：[1]郑州大学细胞生物学研究室,郑州450052

出　　处：《郑州大学学报（医学版）》2004年第1期35-38,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省重大科技攻关基金资助项目 0 12 2 0 3 2 5 0 0 ;河南省杰出人才创新基金资助项目 0 2 2 10 0 190 0

摘　　要：目的 :验证莱茵衣藻叶绿体atpA启动子在杜氏盐藻叶绿体中的转录活性。方法 :以绿色荧光蛋白 (En hancedgreenfluorescentprotein ,EGFP)为报告基因 ,将报告基因插入莱茵衣藻叶绿体atpA启动子与rbcL终止子之间 ,构建载体pSP71 -atpA -EGFP ,用基因枪法转入杜氏盐藻叶绿体中 ,通过荧光显微镜观察EGFP基因在atpA启动子控制下的表达 ,以验证atpA启动子在杜氏盐藻叶绿体中的生物活性。结果 :荧光显微镜下观察到EGFP在杜氏盐藻叶绿体中得到了表达。结论 :莱茵衣藻叶绿体atpA启动子在杜氏盐藻叶绿体中有转录起始活性 。Aim: To prove the transcription activities of the atpA promoter of Chlamydomonas reinhartii chloroplast in Dunaliella salina. Method: The vector pSP71-atpA-EGFP was constructed by inserting the reporter gene ”enhanced green fluorescent protein”(EGFP) between the atpA promoter and the rbcL terminator of chloroplast of Chlamydomonas reinhartii, and transferred to the chloroplast of Dunaliella salina. The expression of EGFP controlled under the atpA promoter was observed through fluorescent microscope to prove the activities of the atpA promoter in Dunaliella salina. Results: The expression of EGFP was observed in the chloroplast of Dunaliella salina under fluorescent microscope. Conclusion: The atpA promoter of the chloroplast of Chlamydomonas reinhartii shows activities in Dunaliella salina, suggesting that it may be used to transform the chloroplast of Dunaliella salina.

关 键 词：盐藻 莱茵衣藻 叶绿体 ATPA 启动子 

分 类 号：Q244[生物学—细胞生物学] Q756