DSPP启动子和DPP基因的突变检测  

Mutation analysis in DPP and DSPP promotor

作　　者：刘嘉利[1] 王晓方[1] 史俊南[1] 张海滨[2] 王英[1] 崔先斌 胡蓝靛[2] 孔祥银[2] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032 [2]中科院生命科学院健康科学中心,上海200025 [3]岳阳市口腔医院,湖南岳阳414000

出　　处：《牙体牙髓牙周病学杂志》2003年第6期301-303,共3页Chinese Journal of Conservative Dentistry

摘　　要：目的 :对 2个遗传性牙本质发育不全Ⅱ型 (DGI-Ⅱ)家系的DSPP启动子和DPP基因编码序列进行突变检测。方法 :在DPP两端设计引物拉出DPP全长 ,以此为模板在DPP序列内部设计引物 ,同时在DSPP启动子内部设计引物 ,经PCR(聚合酶链反应 )和DNA测序进行突变检测。结果 :在DPP内发现插入、缺失等变化 ,但未找到与表型完全一致的突变。结论AIM:To investigate possible gene mutations in DPP coding sequence and DSPP promotor in two Chinese dentinogenesis imperfecta type Ⅱ (DGI-Ⅱ) family.METHODS:PCR and DNA sequencing were employed to detect the possible mutations in DPP coding sequence and DSPP promotor.RESULTS:Insertion and deletion poly- merism were detected in DPP, but no complete segregation with phenotype was observed.CONCLUSION:No mutation was detected in DPP coding sequence and DSPP promotor in these two DGI-Ⅱ families.

关 键 词：牙本质发育不全Ⅱ型 突变 基因 

分 类 号：R780.2[医药卫生—口腔医学]