电化学治疗诱发大鼠种植型肝癌细胞凋亡的实验研究  

Experimental study of electrochemotherapy on implanted liver tumor cells apoptosis of rats

作　　者：邵成伟[1] 王培军[1] 田建明[1] 张火俊[1] 左长京[1] 王敏杰[1] 郝西彦[1] 陈炜[1] 

机构地区：[1]第二军医大学长海医院放射科,上海200433

出　　处：《介入放射学杂志》2003年第2期129-131,共3页Journal of Interventional Radiology

基　　金：国家自然科学基金资助 (3 0 0 70 2 3 3 )

摘　　要：目的　比较电化学治疗对大鼠种植型肝癌细胞凋亡的影响。方法　制作大鼠肝癌动物模型 ,应用电化学疗法进行治疗 ,7d后影像学、病理光镜观察肿瘤大小、坏死变化 ,计算肝肿瘤细胞凋亡指数 ,与对照组比较。结果　电化学治疗前 ,大鼠肝肿瘤平均体积为 (10 0± 6 )mm3 ,电化学治疗后 7d ,大鼠肝肿瘤平均体积为 (12 5± 10 )mm3 ,而荷瘤对照组肝肿瘤平均体积为 (190± 11)mm3 (P <0 .0 5 ) ;治疗组肝肿瘤细胞凋亡指数为 9.6 2 5 %± 1.172 % ,荷瘤对照组细胞凋亡指数为 3.5 2 3%± 0 .894 % (P <0 .0 1) ;治疗组病理检查 ,光镜下肿瘤细胞明显坏死。结论　电化学疗法能诱发细胞凋亡 ,促进肿瘤坏死 。Objective To compare the effects of electrochemotherapy (EChT) on implanted liver tumor cells apoptosis of rats.Methods Immediate cancerous ascitis injection method was used for the formation of rats liver tumor model and then followed by rats liver tumor treatment with EChT. Tumor size, necrosis and apoptosis indexes were observed after one week by imaging or pathologic method. The results were compared with those of control group. Results Before EChT, rats liver tumor volume appeared as (100±6)mm 3 and turned to be (125±10)mm 3 one week after EChT, which were significantly smaller than those of the control group [(190±11)mm 3], P <0.05. Apoetosis indexes were 9.625±1.172 and 3.523±0.894 in EChT group and control group respectively ( P <0.01). Tumor necrosis in EChT group increased significantly than those of the control group.Conclusions The growth of rats liver tumor is obviously inhibited through inducing apoptosis and promoting necrosis by EChT.

关 键 词：电化学治疗 大鼠 种植型肝癌 细胞凋亡 影像学检查 病理特点 

分 类 号：R735.7[医药卫生—肿瘤] R730.56[医药卫生—临床医学]