DNA疫苗的细菌内毒素检测  

Bacterial Endotoxin Test for DNA Vaccine

作　　者：陈祖欢[1] 郭瀛军[1] 章亚男[1] 朱维佳[1] 孙树汉[1] 

机构地区：[1]中国人民解放军第二军医大学基础医学部医学遗传学教研室,上海200433

出　　处：《中国生物工程杂志》2004年第1期81-83,共3页China Biotechnology

摘　　要：目的 :考察DNA疫苗细菌内毒素的检查方法的可行性及DNA疫苗的干扰作用。方法 :干扰试验和对比试验。结果 :供试品阴性对照系列样品溶液无干扰作用 ,与家兔法结果一致。结论 :将疫苗稀释 2倍可用灵敏度为 0 5EUObjective:To establish a bacterial endotoxins test for DNA vaccine using limulus lysate reagent.Method:The bacterial endotoxin test was performed according to the method collected and registered in Chinese Pharmacopeia 2000(Part Two).Results:The bacterial endotoxin test showed that inhibitory effect was not shown when the DNA vaccine was diluted to 6 times,which is consistent with that revealed from the rabbit pyrogen test.Conclusion:The bacterial endotoxin test can be used to detect endotoxin in DNA vaccine.;

关 键 词：DNA疫苗 细菌内毒素 鲎试剂 检测 疫苗制品 

分 类 号：R917[医药卫生—药物分析学]