猪血凝血酶的分离与纯化  

ISOLATION AND PURIFICATION OF PORCINE THROMBIN

作　　者：李敏康[1] 周文静[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西咸阳712081

出　　处：《陕西科技大学学报（自然科学版）》2003年第4期40-42,共3页Journal of Shaanxi University of Science & Technology

摘　　要：报道了以猪血为材料制备凝血酶的新方法:先将猪血浆在-20℃条件下冷冻2d,离心除去纤维蛋白原,再等电点沉淀,得凝血酶原,经CaCl_2激活后,通过DEAE—SepHarose FastFlow离子交换柱层析纯化,获得比活为1807.9U/mg的电泳纯凝血酶制剂,其收率为48.9％。该方法生产工艺简单,所得产品纯度高,对凝血酶的生产具有一定的指导价值。A novel method of isolation & purification of thrombin from the porcine plasma has been developed. The process is as following: the plasma was frozen at - 20C for 2 days, isolating fibrinogen through centrifugation , precipitated thrombinogen at pI. Thrombin was purified by DEAE-Sepharose Fast Flow after activating thrombinogen with CaCl2 and electrophoretic pure was obtained. The recovery of the method was 48. 9% with specific activity 1807. 9U/mg. The method has the property of simple process and high purity of the product, which can be wildly used in manufacture.

关 键 词：猪血 凝血酶 分离 纯化 生产工艺 冷冻-离子交换树脂法 

分 类 号：TQ464.8[化学工程—制药化工] TS251.93[轻工技术与工程—农产品加工及贮藏工程]