胃癌患者HLA-I、B7-1、β_2m的表达  

The expression of HLA-I,B7-1,β_2m moleculars in human gastric carcinoma

作　　者：武焕玲[1] 邹雄[2] 王春霞[1] 车至香[1] 李桂琴[1] 

机构地区：[1]山东省立医院,山东济南250021 [2]山东大学医学院

出　　处：《中国实验诊断学》2004年第1期49-52,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的　探讨HLA I、β2 m、B7 1分子在肿瘤发生、发展中的作用。方法　流式细胞技术检测胃肿瘤细胞和外周血淋巴细胞HLA I、β2 m、B7 1分子的表达 ;放射免疫技术 (RIA)检测血清中 β2 m的含量。结果　①胃癌细胞表面HLA I分子表达明显降低。②胃癌细胞表面B7 1分子表达明显降低或缺如。③β2 m在肿瘤细胞上的表达明显增高 ;作相关分析发现 ,β2 m与HLA I的表达呈负相关关系 (r=- 0 4 70 ,P <0 0 5 )。④在外周血淋巴细胞上各指标的表达情况 ,HLA I表达丰富 ,胃癌患者与正常人之间无显著性差异 ;B7 1的表达微弱 ,胃癌患者的表达低于正常人 ;β2 m的表达丰富 ,胃癌患者的表达高于正常人 ;⑤血清中 β2 m的含量 ,胃癌患者高于正常人 ,血清中 β2 m的含量与外周血淋巴细胞β2 m表达有正相关关系 (r=0 5 4 6 ,P <0 0 5 )。结论　肿瘤细胞低表达HLA I和B7分子是肿瘤发生、发展的重要机制 ;β2 m在肿瘤的诊断、治疗和预后判断有一定价值。Objective To study the roles of HLA-I,B7-1 and β 2m moleculars in gastric carcinoma's occurrence and development. Methods FCM were performed to analyse HLA-I,B7-1 and β 2m moleculars on gastric carcinoma cells and peripheral lymphocytes; radiation immunological method were perfomed to analyse β 2m in serum. Results ①The expression of HLA-I moleculars on gastric carcinoma cells decreased dramatically when compared with normals; ②The expression of B7-1 moleculars on gastric carcinoma cells was even weaker. ③β 2m was much higher in gastric carcinoma cells. Then the correlation of β2m and HLA-I on issue cells analyse.Conclusion There are negitive correlated(r=-0.470,P<0.05). ④On lympocytes, HLA-I was expressed plentifully, no statistic differences were found between gastric carcinoma patients and the normal; B7-1 was still weakly expressed on lympocytes, there was difference between gastric carcinoma patients and the normal. ⑤The quantity of β 2m in serum of gastric carcinoma patients was higher than the normal and was related to β 2m expression on peripherical lymphocytes.

关 键 词：胃癌 HLA-I B7-1 Β2M 肿瘤 诊断 治疗 

分 类 号：R735.2[医药卫生—肿瘤]