rhBMP-2对人牙龈成纤维细胞体外矿化能力的影响  

The Effects of Recombinant Human Bone Morphogenetic Protein-2 on the Mineralization Capability of Human Gingival Fibroblasts in Vitro.

作　　者：张韶君[1] 彭凤梅[2] 章锦才[3] 张蕴惠[1] 

机构地区：[1]四川大学华西口腔医学院,四川成都610041 [2]山东省千佛山医院口腔科 [3]广东省口腔医院

出　　处：《广东牙病防治》2004年第1期15-17,81,共4页Journal of Dental Prevention and Treatment

基　　金：国家自然科学基金资助项目 (39970 793)

摘　　要：目的　探讨重组人骨形成蛋白 2 (rhBMP 2 )对人牙龈成纤维细胞 (GF)体外矿化能力的影响。方法　以组织块培养法培养人牙龈成纤维细胞 ,分别以含或不含rhBMP 2的矿化培养液长期培养 ,以倒置显微镜观察细胞生长状况。于培养第 7天检测碱性磷酸酶 (ALP)活性。于培养第 30天行vonKossa染色观察矿化结节。结果　rhBMP 2提高GF的ALP水平 ,呈剂量依赖性 ,并能够刺激GF在体外形成矿化结节。结论　rhBMP 2能够诱导人GF向成骨细胞表型分化。Objective To investigate the effects of recombinant human bone morphogenetic protein-2(rhBMP-2) on the mineralization capability of human gingival fibroblasts (GF) in vitro. Methods Human gingival fibroblasts (GF) were primary cultured through tissue culture method and then mineralized cultured with or without rhBMP-2 within a period of given time. The effects of rhBMP-2 on alkaline phosphatase (ALP) activity and formation of mineralized nodule were detected. Results RhBMP-2 siganifcantly increased the ALP activity in a dose dependent manner,and stimulated the mineralized nodule formation of GF in vitro. Conclusion RhBMP-2 could stimulate the expression of oateogenetic phenotype of GF in vitro.

关 键 词：RHBMP-2 牙龈 成纤维细胞 体外矿化能力 人骨形成蛋白-2 牙周病 

分 类 号：R781[医药卫生—口腔医学]