重组水蛭素Ⅲ的工程菌的培养及高密度发酵  

Cultivation and High-Density Fermentation of Recombinant Hirudin Ⅲ in Engineered E.coli

作　　者：崔莉[1] 谭树华[1] 吴梧桐[1] 

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京210009

出　　处：《药物生物技术》2004年第1期22-24,共3页Pharmaceutical Biotechnology

基　　金：国家自然科学基金资助;No .3 0 0 0 0 2 14;国家教育部霍英东教育基金会青年教师基金项目No .810 3 2

摘　　要：为提高重组水蛭素Ⅲ工程菌的产水蛭素量 ,采用正交实验法确定了较佳培养基组分 ,优化了摇瓶发酵工艺和发酵条件 ,工程菌摇瓶水蛭素产量稳定在 2 0 0 0ATU/ml左右 ,30L发酵罐批式发酵水蛭素产量达到4 0 0 0ATU/ml,补料 -批式发酵水蛭素产量达到 80 0 0ATU/ml。In order to improve the hirudin production by an engineered Escherichia coli, a suitable culture medium was selected by orthogonal experimental design, some fermentation conditions were optimized. Hirudin was maintained 2 000 ATU/ml in flask and 4 000 ATU/ml in a 30 L fermentor. However, hirudin was further enhanced to 8 000 ATU/ml in a 30 L fed-batch fermentor.

关 键 词：水蛭素Ⅲ 大肠杆菌 发酵 

分 类 号：Q78[生物学—分子生物学] TQ920[轻工技术与工程—发酵工程]