苦参碱对豚鼠心肌电生理及机械特性的影响  

Effects of matrine on action potentials and contractile force in guinea pig papillary muscles

作　　者：谢华[1] 温洪涛[1] 高远[1] 胡香杰[1] 张贵卿[1] 

机构地区：[1]郑州大学医学院药理学教研室,郑州450052

出　　处：《中国药理学通报》2004年第2期192-195,共4页Chinese Pharmacological Bulletin

摘　　要：目的　观察苦参碱对离体豚鼠右心室乳头状肌动作电位及收缩力 (Fc)的影响 ,以探讨其抗心律失常的作用机制。方法　采用标准玻璃微电极技术记录心肌细胞动作电位 ,肌力换能器同步记录心肌收缩力。结果　苦参碱 10、2 5、5 0 μmol·L-1可浓度依赖性地延长快反应动作电位(FAP)的复极 5 0 %时程 (APD50 )、复极 90 %时程 (APD90 )和有效不应期 (ERP) ,延长高钾除极组织胺及氯化钡诱发的慢反应动作电位 (SAP)的APD50 、APD90 ;但对FAP、SAP的动作电位振幅 (APA)、0期最大除极速率 (Vmax)及Fc无明显影响。结论　结果提示苦参碱对心肌细胞钠、钙离子通道无明显影响 ,其延长APD的作用可能是阻断心肌钾通道的结果。AIM To study the effects of matrine (Ma) on the action potential and contractile force in guinea pig papillary muscles. METHODS Conventional microelectrode technique was used to record the fast action potentials (FAP) and slow action potentials (SAP) induced by histamine and BaCl_2 of guinea pig papillary muscles. RESULTS Ma 10,25,50 μmol·L -1 dose-dependently prolonged the action potential duration at 50%, 90% repolarization (APD_ 50 , APD_ 90 ) and effective refractory period (ERP) of FAP, and lengthened the APD_ 50 , APD_ 90 of SAP induced by histamine and BaCl_2 when perfused with KCl 25 mmol·L -1 Tyrode's solution. The maximal upstroke velocity (V_ max ) of FAP, SAP and contractile force (Fc) were not affected by matrine 10,25,50 μmol·L -1 . CONCLUSION It was suggested that Matrine could block K + channels in the myocardium.-

关 键 词：苦参碱 乳头状肌 动作电位 有效不应期 心肌收缩力 

分 类 号：R-332[医药卫生] R282.71