Variability of cell proliferation in the proximal and distal colon of normal rats and rats with dimethylhydrazine induced carcinogenesis  

作　　者：Kate E Williamson Brian J Rowlands 

机构地区：[1]Department of Surgery,Institute of Clinical Science,The Queen's University of Belfast,Belfast,UK

出　　处：《World Journal of Gastroenterology》2002年第5期847-852,共6页世界胃肠病学杂志（英文版）

基　　金：in part by DHSS of Northern Ireland.

摘　　要：AIM：To investigate the patterns of cell proliferation in proximal and distal colons in normal rats and rats with1,2-dimethylhydrazine(DMH)induced carcinogenesis using the thymidine analogue bromodeoxyuridine.METHODS:Colonic crypt cell proliferation was immunohistochemically detected using the anti-bromodeoxyuridine Bu20a monoclonal antibody.RESULTS:Marked regional differences were found in both groups.Total labelling index(LI)and proliferative zone size in both normal(8.65±0.34vs7.2±0.45,27.74±1.07vs16.75±1.45)andDMH groups(13.13±0.46vs11.55±0.45,39.60±1.32vs35.52±1.58)were significantly higher in distal than in proximal colon(P<0.05).although the number of cells per proxmal crypt was greater(31.45±0.20vs34.45±0.39,42.68±0.53vs49.09±0.65,P<0.001).Crypt length,total LT and proliferative zone size all increased in both proximal and distal regions of DMH rats compared to normal controls(P<0.0001).In DMH-treated rat colon a shift of labelled cells to higher crypt cell positions was demonstrated distally whist a bi-directional shift was evident proximally(P<0.05).CONCLUSION:Our results show that changes in cell proliferation patterns,as assessed by bromodeoxyuridine uptake,can act as a reliable intermediate marker of colonic cancer formation.Observed differences between proliferation patterns in distal and proximal colon may be associated with the higher incidence of tumors in t he distal colon.AIM:To investigate the patterns of cell proliferation in proximal and distal colons in normal rats and rats with 1,2- dimethylhydrazine(DMH)induced carcinogenesis using the thymidine analogue bromodeoxyuridine. METHODS:Colonic crypt cell proliferation was immunohistochemically detected using the anti- bromodeoxyuridine Bu20a monoclonal antibody. RESULTS:Marked regional differences were found in both groups.Total labelling index(LI)and proliferative zone size in both normal(8.65±0.34 vs 7.2±0.45,27.74±1.07 vs 16.75±1.45)and DMH groups(13.13±0.46 vs 11.55±0.45, 39.60±1.32 vs35.52±1.58)were significantly higher in distal than in proximal colon(P<0.05),although the number of cells per proximal crypt was greater(31.45±0.20 vs 34.45 ±0.39,42.68±0.53 vs49.09±0.65,P<0.0001).Crypt length, total LI and proliferative zone size all increased in both proximal and distal regions of DMH rats compared to normal controls (P<0.0001).In DMH-treated rat colon a shift of labelled cells to higher crypt cell positions was demonstrated distally whilst a bi-directional shift was evident proximally(P<0.05).CONCLUSION: Our results show that changes in cell proliferation patterns, as assessed by bromodeoxyuridine uptake, can act as a reliable intermediate marker of colonic cancer formation. Observed differences between proliferation patterns in distal and proximal colon may be associated with the higher incidence of tumors in the distal colon.

关 键 词：溴脱氧尿嘧啶 二甲肼 细胞增殖 致癌机制 动物实验 

分 类 号：R574[医药卫生—消化系统] R735.3[医药卫生—内科学]