分光光度法测定显齿蛇葡萄总黄酮含量  

Determination on Total Flavanoids of Ampelopsis grossedentata by Spectrophotometry

作　　者：熊皓平[1] 杨伟丽[1] 何国庆[2] 张友胜[3] 

机构地区：[1]湖南农业大学食品科技学院,湖南长沙410128 [2]浙江大学生物系统工程与食品科学院,浙江杭州310029 [3]广东省农业科学院蚕业研究所,广东广州510640

出　　处：《食品科学》2004年第2期144-145,共2页Food Science

摘　　要：用95%的乙醇为溶剂连续加热回流浸提显齿蛇葡萄幼嫩茎叶中有效成分,以二氢杨梅素为标样,选择波长294nm,采用三氯化铝分光光度法,建立显齿蛇葡萄总黄酮含量测定方法。实验结果表明:标液浓度在5～50μg/ml范围内,吸光度与浓度呈良好的线性关系,回归方程为A=3.166×10-3X-9.0×10-3,r=0.9999(n=5)。加样平均回收率为98.6%,RSD为2.54%。本法操作简便、快速、准确,可作为该药材质量评价方法。A method for the determination on total flavanoids of Ampelopsis grossedentata was established: using 95% ethanolby continuous thermal reflux to extract its young shoots, taking dihydromyricetin as standard sample, selecting 294nm wave-length and by AlCl3 spectrometric method. The results showed that the regression equation, within the range of the standard sampleconcentration in 5-50μg/ml, was A=3.166×10-3X-9.0×10-3, with a good linearity(r=0.9999)between the absorbance andconcentration. The average recovery rate was 98.6% and RSD was 2.54% (n=5). The method is simple, rapid and accurate andsuitable for the quality evaluation of Ampelopsis grossedentata.

关 键 词：分光光度法 测定方法 显齿蛇葡萄 总黄酮 有效成分 二氢杨梅素 

分 类 号：R284[医药卫生—中药学]