STR位点基因扫描在亲子鉴定中的应用  

The application of STR loci genescan in paternity testing

作　　者：余兵[1] 秦群霞[2] 李昌峰[3] 李生斌[1] 

机构地区：[1]西安交通大学医学院部级法医学重点实验室 [2]西安交通大学第一医院中医科,陕西西安710061 [3]中国科学院遗传研究所,北京100101

出　　处：《西安交通大学学报（医学版）》2004年第1期24-26,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助 (No .36 970 399)

摘　　要：目的　探索一种易于标准化的亲子鉴定方法。方法　选用九个群体家系资料明确的四核苷酸重复的STR位点 ,用荧光标记物标记引物、PCR复合扩增、灵敏度更高的 3 77测序仪对 45例亲子鉴定案例进行电泳分型 ,并自动收集数据和分析 ,自动判定STR位点等位基因和基因型。结果　 2 1个案例排除亲子关系 ,2 4例不排除亲子关系。结论　STR位点的基因扫描方法简便、省时、快速 ,灵敏度高 。Objective To search for an easy standardization p aternity testing method. Methods Based on nine completed da ta and stable tetranucleotide STR loci, 45 paternity cases were typed by using f luorescence labeling primer, multiplex PCR, higher sensitivity 377 DNA sequencer , and then the computer automatically collected the data and made analysis, STR loci alleles and genotypes were determined. Results Th ere was no relationship between alleged fathers and children in 21 cases. But in 24 cases the relationship could not be excluded. Conclusion The STR loci genescan method is convenient, time-saving, fast and has hig her sensitivity. The results are accurate and reliable.

关 键 词：STR位点 基因扫描 亲子鉴定 父权指数 遗传标记 

分 类 号：R394[医药卫生—医学遗传学]