胶质细胞源性神经营养因子对脊髓前角运动神经元的保护作用(英文)  

Protective reaction of glial cell line-derived neurotrophic factor to anterior cornual motoneuron

作　　者：宋海涛 贾连顺[2] 田万成 陈哲宇[3] 何成[3] 

机构地区：[1]解放军第107中心医院济南军区创伤骨科中心,山东省烟台市264002 [2]解放军第二军医大学附属长征医院骨科,上海市200003 [3]解放军第二军医大学神经科学研究所,上海市200433

出　　处：《中国临床康复》2004年第4期788-789,共2页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(39500044)~~

摘　　要：目的:证实胶质源性神经营养因子(glialcellline-derivedneurotrophicfactor,GDNF)对脊髓前角运动神经元有保护作用。方法:切断SD大鼠一侧坐骨神经建立脊髓前角运动神经元损伤模型,借助单盲端硅胶管系统在损伤神经局部给予GDNF,术后不同时间分别取L5脊髓切片,利用酶组织化学染色方法显示胆碱酯酶和酸性磷酸酶(acidphosphatase,ACP)活性并进行图像分析。结果:坐骨神经切断后第4,9,16及21d,对照组和GDNF组胆碱酯酶活性分别为21.53±1.54和23.67±1.08(P<0.05),19.82±1.35和22.87±1.04(P<0.01),22.55±1.20和25.25±1.13(P<0.01),25.52±1.43和28.92±1.37(P<0.01);对照组和GDNF组ACP活性(平均灰度)分别为37.49±1.39和35.40±1.18(P<0.05),40.94±1.38和38.43±1.31(P<0.05),22.55±1.20和25.25±1.13(P<0.05),37.15±1.52和34.68±1.43(P<0.05)结论:GDNF对损伤的脊髓前角运动神经元有保护作用。AIM:To verify the protective reaction of glial cell line-derived neruotroph ic factor(GDNF) to anterior cornual motoneuron. METHODS:Anterior cornual motoneuron injury model was established by disjuncti on one side of sciatic nerve in SD rat.GDNF was provided locally at neuron focal area by dint of mono-cecum silicon vessel system.L5 spinal cord slice was tak en at different time after surgery.The activities of Choline esterase and acid p hosphatase(ACP) was displayed by enzymic histochemical coloring method and analy zed through image assay. RESULTS:On the 4th,9th,16th,and 21st day after the disjunction of sciatic ner ve,the activities of choline esterase in control and GDNF groups were 21.53± 1. 54 and 23.67± 1.08 respectively(P< 0.05),19.82± 1.35 and 22.87± 1.04(P< 0.01) ,22.55± 1.20 and 25.25± 1.13(P< 0.01),and 25.52± 1.43 and 28.92± 1.37 (P< 0. 01) respectively.ACP activities(mean gray,MG) in control and GDNF groups were 37 .49± 1.39 and 35.40± 1.18(P< 0.05),40.94± 1.38 and 38.43± 1.31(P< 0.05),22.5 5± 1.20 and 25.25± 1.13(P< 0.05),and 37.15± 1.52 and 34.68± 1.43(P< 0.05) re spectively. CONCLUSION:GDFN has protective reactions to anterior cornual motoneuron injur y.

关 键 词：胶质细胞源性神经营养因子 脊髓前角 运动神经元 CDNF 坐骨神经 

分 类 号：R741[医药卫生—神经病学与精神病学]