外源高表达PC-1蛋白N端43个氨基酸可加速人前列腺癌细胞系C4-2的生长  

Exogenous excessive expression of PC-1 gene N terminus 43 amino acids can promote the growth of human prostate cancer line C4-2

作　　者：万里川[1] 周建光[1] 李杰之[1] 孙玉龙[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100850

出　　处：《生物技术通讯》2004年第1期1-4,共4页Letters in Biotechnology

基　　金：国家自然科学基金(30070296)

摘　　要：研究PC-1蛋白N端43个氨基酸表达对人前列腺癌细胞C4-2生长的影响。用DNA重组技术将含PC-1蛋白N端43个氨基酸的DNA序列正向克隆到真核表达载体pIRES2-EGFP中,采用脂质体法将重组质粒稳定转染进C4-2细胞中,RT-PCR分析外源序列的转录情况,固相ELISA法测定PC-1蛋白N端43个氨基酸的表达,MTT实验分析细胞的生长速度。结果获得了稳定转染PC-1基因N端43个氨基酸的前列腺癌细胞株,在该细胞株中外源PC-1蛋白N端43个氨基酸得到高表达,细胞生长速度较对照细胞加快了38%。结果表明外源PC-1基因N端43个氨基酸高表达可提高人前列腺癌细胞C4-2的生长速度,推论PC-1基因高表达可能在人前列腺癌的发展中起一定的促进作用。To study the effect of exogenous excessive expression of PC-1gene N terminus43amino acids to the growth of human prostate cancer cell line C4-2.Using DNA recombinant technology,N-terminus43amino acids of PC-1gene was inserted into eukaryotic expression vector pIRES2-EGFP.The recombinant plasmid was transfected into C4-2cell via liposome.The transcription of the exogenous sequence was examined by reverse transcriptase polymerase chain reaction.Solid phase ELISA was utilized to detect the translation of endogenous N-terminus43amino acids of PC-1protein.Growth rate was observed by MTT experiment.RT-PCR demonstrated that exogenous sequence has been transcripted in C4-2cell.In addition,N terminus43amino acids of PC-1protein has been translated.Furthermore,MTT experiment showed growth rate of C4-2cell increased38%after transfection.The result suggested that exogenous excessive expression of N terminus43amino acids of PC-1gene could increase the growth rate of human prostate cancer cell line C4-2.It could extrapolate that PC-1gene overexpression might play a promotion role in the progression of prostate cancer.

关 键 词：前列腺癌 基因表达 生长速度 稳定转染 

分 类 号：R737.25[医药卫生—肿瘤]