大蹼铃蟾抗菌肽-2与IFN-α-2b和TNF-α对膀胱癌细胞株抑制活性的实验研究  

Control Experiment of Bombina Maxima Antimicrobial Peptides 2 and TNF-α,IFN-α-2b on Bladder Cancer Cell Lines Growth

作　　者：申吉泓[1] 张建华[1] 官润云[1] 李文辉[2] 李响[3] 杨宇如[3] 张云[2] 

机构地区：[1]昆明医学院第一附属医院泌尿外科,云南昆明650032 [2]中国科学院昆明动物研究所"知识创新工程"基地"动物活性蛋白多肽组学"实验室,云南昆明650032 [3]四川大学华西医院泌尿外科,四川成都610041

出　　处：《肿瘤防治杂志》2003年第5期459-462,共4页China Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金资助项目 (30 1 70 1 95);中国科学院知识创新工程

摘　　要：目的 :比较大蹼铃蟾抗菌肽 - 2 (Maximin 2 )与肿瘤坏死因子 α(TNF α)、干扰素 α 2b(IFN α 2b)对不同膀胱癌细胞株的抑制杀伤作用。方法 :采用细胞培养及MTT比色法 ,检测Maximin 2、TNF α及IFN α 2b对 3个不同膀胱癌细胞株的抑制作用及作用特点 ,计算量效回归曲线及 5 0 %抑制率。结果 :Maximin 2对不同的膀胱癌细胞株在 10 0 μg mL左右 (4 8h)即达到 5 0 %抑制率浓度 (IC50 ) ,并呈剂量相关性 ,与TNF α、IFN α 2b抑制作用的表现形式不同。Maximin 2抑制率最高表现在第 4 8h ,以后逐渐减弱 ,TNF α、IFN α 2b则在前 2 4h作用较弱 ,以后逐渐增强。结论 :Maximin 2对不同的膀胱癌细胞株均有较强的抑制作用 ,同TNF α、IFN α 2b比较其作用机制有所不同 ,Maximin 2对膀胱癌细胞株的杀伤作用可能主要是通过对肿瘤细胞的直接抑制 。Objective To compare the effects of Maximin 2 with TNF α and IFN α 2b on three differrent kinds of bladder cancer cell lines.Methods Cell culture and MTT assay were used to dectect the effects of Maximin 2,TNF α and IFN α 2b on three different bladder cancer cell lines on cell growth,calculating 50%inhibitory rate of differrent bladder cell lines by concentration dosage and regression equation.Results Maximin 2 suppress the vitro growth in a dose and time dependent manner and the IC 50 was about 100 μg/mL(48 hours),compared with that of TNF α,IFN α 2b the appearance was different.Maximin 2 was more effective in first 48 hours.However,the inhibitory rate of TNF α,IFN α 2b was minor in the first 24 hours and was intensive with time extending.Conclusions Although Maximin 2 shows the effect on restrain cell lines growth related to the dosage,compared with TNF α and IFN α 2b,the major mechanism is different.Maximin 2 inhibiteing cancer cell growth may result from direct inhibition on cancer cell. China J Cancer Prev Treat,2003,10(5):459-462

关 键 词：膀胱癌 膀胱肿瘤 治疗 抗肿瘤药 药理学 蟾蜍科 大蹼铃蟾 抗菌肽-2 IFN-Α-2B TNF-Α 肿瘤抑制 

分 类 号：R737.14[医药卫生—肿瘤]