二种酵母多糖的纯化、鉴定及抗氧化性质分析  

Purification, Identification and Anti-oxidative Analysis of Two Yeast Polysaccharides

作　　者：王义华[1] 徐梅珍[2] 江萍[3] 何照范[3] 熊绿芸[3] 

机构地区：[1]清华大学生物科学与技术系,北京100094 [2]江西农业大学食品科学系,江西南昌330045 [3]贵州大学生物技术学院,贵州贵阳550025

出　　处：《食品科学》2004年第3期33-36,共4页Food Science

基　　金：江西省科技厅农业攻关项目(2003123600);江西省自然科学青年基金(0330061)

摘　　要：以热带假丝酵母和八孢裂殖酵母为材料,通过化学抽提法得到水溶性提取物Mb与Mc;经薄层层析分析表明:Mb主要含有甘露糖,Mc含有甘露糖和半乳糖;经检测,Mb和Mc含有蛋白质,为蛋白多糖;Mb和Mc中氨基酸种类丰富,其中含有7种必需氨基酸和9种非必需氨基酸;邻苯三酚自氧化法发现多糖能够抑制邻苯三酚自氧化,具有体外抗氧化能力。Two soluble extractions of polysaccharides named Mb and Mc were extracted from Candida tropicalis andSchizosacchromyces octosporus by chemical precipitation method. Thin layer chromatagrathy showed that Mb was mainlycomposed of mannase and Mc was composed of mannase and galactose. Protein analysis revealed that Mb and Mc consisted ofpolysacchrides and proteins and amino acid analysis proved that Ma1 contained 7 kinds of essential and 9 kinds of non-essentialamino-acids. Pyrogallol autooxidation analysis revealed that Mb and Mc showed anti-oxidative properties in vitro.

关 键 词：酵母多糖 纯化 鉴定 抗氧化 Mb Mc 薄层层析 热带假丝酵母 八孢裂殖酵母 

分 类 号：Q936[生物学—微生物学]