K562白血病细胞系HLA-II等位基因型  

Study on Genotype of HLA-II Alleles in K562 Cell Lines

作　　者：谢桂岚[1] 李志祥[2] 

机构地区：[1]首都医科大学附属北京友谊医院血液内科,北京100050 [2]首都医科大学附属北京友谊医院移植配型中心,北京100050

出　　处：《天津医药》2004年第3期142-144,共3页Tianjin Medical Journal

摘　　要：目的:测定K562白血病细胞系HLA -II等位基因型。方法:采用聚合酶链反应 -限制性片断长度多态性技术对K562白血病细胞系HLA -II类抗原基因进行DNA分型。结果:K562细胞系的HLA -II基因型均为纯合子型 ,等位基因分别是 :DRB1*0301/*0301,DQB1*0201/*0201,DPB1*0402/*0402。结论:提高对K562白血病细胞系HLA -II基因型的认识。Objective:To study the genotype of HLA_II alleles in K562 cell lines.Methods:The HLA_II alleles in K562 cell lines were investigated with polymerase chain reaction-restriction fragment length polymorphism(PCR-RELP)method.Results:The HLA-II alleles in K562 cell lines were DRB1*0301/*0301,DQB1*0201/*0201 and DPB1*0402*0402 respectively.All genotype were homozygote.Conclusion:This study is important for understanding immunogenetics of acute myelogehous leukemia.

关 键 词：K562白血病细胞系 HLA—Ⅱ 等位基因型 细胞培养 PCR—RFLP方法 

分 类 号：R730.21[医药卫生—肿瘤]