产L-乳酸米根霉PW352特性及低能离子注入诱变高产菌株研究  

Characteristics of L-lactic acid producing strain Rhizopus oryzae PW352 and high productive mutant by low energy ions implantation

作　　者：古绍彬[1] 葛春梅[1] 汪青宏[2] 张洁[2] 姚建铭[1] 潘仁瑞[1] 余增亮[1] 

机构地区：[1]中国科学院等离子体物理研究所离子束生物工程重点实验室,合肥230031 [2]合肥联合大学生物工程教研室,合肥230022

出　　处：《工业微生物》2004年第1期12-16,共5页Industrial Microbiology

基　　金：国家十五攻关项目 ( 2 0 0 1BA3 0 2B)

摘　　要：为了获得更适于工业生产的产乳酸菌株 ,以葡萄糖为碳源对PW35 2菌株特性进行了研究 ,并在此基础上采用离子束诱变方法 ,对米根霉PW 35 2进行改良 ,获得高产L(+) 乳酸菌株RE330 3,产酸能力达 131～ 136 g L ,最高可达 14 0g L ,糖转化率为 86 %～ 90 % ,产酸比PW 35 2提高 75 %。In order to obtain higher L-lactic acid yield industrial strain, the characteristics and fermentation conditions of Rhizopus oryzae PW352 using glucose as carbon source were studied. The original strain PW352 was mutated by means of N + ions implantation and a mutant strain RE3303 was obtained. Its lactic acid production ability was increased by 75% than that of the original. The concentration of L-lactic acid reached to 131～136g/L and the conversion rate of glucose was as high as 86%～90% under the condition of 150g/L glucose in fermentation medium.

关 键 词：离子注入 L(+)-乳酸 根霉 诱变 

分 类 号：TS252[轻工技术与工程—农产品加工及贮藏工程]