耐热重组β-半乳糖苷酶的制备及酶稳定性研究  

Preparation and stability of thermstable β-galactosidase from recombinant Bacillus subtilis

作　　者：杨静[1] 陈卫[1] 傅晓燕[1] 张灏[1] 

机构地区：[1]江南大学食品学院,江苏无锡214036

出　　处：《工业微生物》2004年第1期35-38,共4页Industrial Microbiology

摘　　要：编码嗜热脂肪芽孢杆菌 β -半乳糖苷酶基因的重组枯草芽孢杆菌WB6 0 0 pMA5 bgaB ,经 5L发酵罐发酵收集菌体超声波破壁 ,冷冻离心后再经过热处理和盐析纯化 ,比活力达 80 .3U mgpr ,回收率 73.6 % ,纯化倍数 12 .4倍。β -半乳糖苷酶在细胞内及破壁后都具有较好的稳定性 ,6The thermostable β-galactosidase was released from the recombinant Bacillus subtilis WB600/pMA5-bgaB by ultrasonic. The crude enzyme was purified for 12.4 folds with the specific activity as high as 80.3 U/mg protein by heat treatment and ammonium sulfate fractionation. The thermostable β-galactosidase within the cells or in the buffer was relatively stable. And the heat treatment below 60℃ had less influence on the stability of enzyme conformation.

关 键 词：超声波 热处理 盐析 稳定性 β—半乳糖苷酶 嗜热脂肪芽孢杆菌 

分 类 号：TQ925[轻工技术与工程—发酵工程]