HPLC-PITC衍生法测定灌流液中氨基酸变化的研究  

Study of Free Animo Acids in Infused Fluid of Hippocampus by HPLC-PITC

作　　者：赵刚[1] 张嘉麟[1] 李树清[1] 

机构地区：[1]昆明医学院病理生理学教研室,昆明650031

出　　处：《昆明医学院学报》2004年第1期26-28,共3页Journal of Kunming Medical College

基　　金：云南省自然科学基金资助课题 (2 0 0 1C0 0 6 4M)

摘　　要：目的 :建立一种高效液相色谱法以检测树鼠句 海马灌流液中Glu ,Asp ,Gly和GABA的含量 .方法 :色谱柱 :WatersNovapakC18;流动相 :A液 ,NaAC·3H2 O 19 0g ,TEA 0 5mL ,用双蒸水稀释至 1L后 ,按 94∶6加入乙腈 ;B液 :乙腈∶超纯水 =3∶2 (V/V) ,梯度洗脱 ;检测器 ,紫外 2 5 4nm ;灌流液样品经蛋白沉淀、干燥、衍生、真空再干燥和氨基酸稀释液溶解后进样 .结果 :上述各氨基酸分离良好 ,色谱峰保留时间分别为 1 6 5 ,3 2 7,4 0 0和 10 35min ,脑缺血后出现的变化具有显著差异 (P <0 0 1) .结论 :该法灵敏、快速而有效 。Objective: To research a kind of high-performance liquid chromatography (HPLC)-PITC technology in order to detect the level of Asp, Glu, Gly and GABA in infused fluid of hippocampus in tree shrews. Methods: Waters Novapak C18 (300?mm×15?mm); Mobile phases is consisted of A and B. Detecter UV 254?nm. Results: The above amino acids are separated very well and contained at 1.65 min, 4.00?min and 10.35?min respectively. There are significant differences between the ischemic and sham (P<0.01). Conclusion: The method of HPLC-PITC is fast, sensitive and effective in the monitor of the free amino acids from central nervous system.

关 键 词：HPLC-PITC衍生法 测定 灌流液 氨基酸 

分 类 号：R363.2[医药卫生—病理学]