小麂Sry基因的克隆和测序  

Cloning and Sequencing of Sry Gene of Muntiacus

作　　者：鲁晓瑄[1] 张悦[1] 单祥年[1] 

机构地区：[1]东南大学医学院遗传学研究中心,南京210009

出　　处：《遗传》2003年第3期299-301,共3页Hereditas（Beijing)

基　　金：国家自然科学基金资助课题(39670393)

摘　　要：应用人的性别决定基因SRY(Sex determiningRegionYgene,SRY)中HMG框内的一对引物,对小麂细胞株的基因组DNA进行PCR扩增,得到雄性小麂细胞的220bp扩增产物,而在雌性小麂细胞中未发现扩增产物。将雄性小麂细胞的220bp扩增产物通过T-A互补法克隆到质粒pGEM-T载体中,筛选阳性克隆进行DNA测序。测序结果表明小麂Sry基因保守序列与人的SRY基因保守区相同碱基的比值为152/184,达到82.6%。提示小麂Sry基因与人的SRY基因存在着较高的同源性,说明SRY基因在进化过程中高度保守。Using the primers from SRY gene--HMG Box for PCR amplification in genomic DNA of Muntiacus reevesi cell strains,a 220bp fragment was obtained in the male but not in the female.The 220bp fragment was cloned into the pGEM-T vector using T/A clone method.The identified positive clone was sequenced.The result shows that 82.6% nucleotides(152bp/184bp) are homologous between Muntiacus Sry and human SRY gene.It suggests that SRY is highly conserved during evolution.

关 键 词：SRY基因 性别决定 小麂 克隆 DNA 人 同源性 序列分析 

分 类 号：Q953[生物学—动物学]