人肺癌细胞γ-干扰素基因的转染及特性分析  

Characterization of human lung cancer cell line A549 transfected with human interferon-gamma gene

作　　者：于晓虹[1] 刘祥麟[1] 马洁羽[1] 

机构地区：[1]浙江大学医学院,浙江杭州310031

出　　处：《浙江大学学报（医学版）》2004年第2期125-128,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省科技厅科研基金 (2 0 0 330 0 1);杭州市科委研究基金 (99113A0 7);杭州市卫生局研究基金 (招 980 1)资助项目

摘　　要：目的 :建立转基因 A5 49细胞株 ,从特性上进行分析 ,探讨其作为抗肿瘤瘤苗的可能性。方法 :将 p L XSN质粒与人 IFN-γ基因体外重组 ,转染入人肺腺癌细胞株 A5 49中 ,经筛选获得稳定表达γ-干扰素的 A5 49- IFN-γ细胞株 ,对其生长特性、细胞表面 MHC分子表达变化、IFN-γ的分泌量及致瘤性等方面进行了探讨。结果 :转基因细胞株与原细胞株相比较 ,生长状态没有明显的变化 ,转基因细胞株可稳定表达并向细胞外分泌γ-干扰素 ;而且其细胞表面 MHC- 和 MHC- 分子表达明显增加 ,提示其免疫原性增强 ;裸鼠实验表明其致瘤性下降。结论 :该转基因细胞株有可能成为治疗肺癌的转基因瘤苗。Objective: To establish a human lung cancer cell line expressing human interferon-gamma. Methods: The full-length gene of human interferon-gamma (IFN-γ) was introduced into the human lung adenocarcinoma cell line A549 through retroviral vector pLXSN. The established cell line A549-IFN-γ was tested for expression of MHC class Ⅰ and class Ⅱ by flow cytometer (FCM) and tested for expression of IFN-γ by enzyme-lined immunoadsorbent assay (ELISA ). The tumorigenesis of cell line A549-IFN-γ was tested on nude mice. Result: A high level of IFN-γ protein was detected in the culture supernatants of cell line A549-IFN-γ. The expressions of MHC class Ⅰ and class Ⅱ on A549-IFN-γ cells increased significantly (P<0.01), when compared with parental cell line A549. However, there was no significant difference (P>0.05) between the growth of cell line A549-IFN -γ and A549. Finally, the tumorigensis test showed that A549-IFN-γ had lower tumorigenetic effects than A549. Conclusion: The results indicate that introduction of human IFN-γ gene into cell line A549 could increase its immunogenicity and decrease its tumorigenesis. With the established cell line A549-IFN-γ, a tumor vaccine for human lung cancer may be developed.

关 键 词：干扰素Ⅱ型/遗传学 肺肿瘤/治疗 γ-干扰素/pLXSN 主要组织相容性复合物 人肺腺癌细胞株 

分 类 号：R734.2[医药卫生—肿瘤]