里氏木霉β-葡萄糖苷酶基因的克隆及序列分析  

The gene clone and sequence analysis of bglI from Trichoderma reesei

作　　者：张彬彬[1] 李杰[1] 柏锡[1] 丁晓东[1] 朱延明[1] 

机构地区：[1]东北农业大学生命科学学院植物生物工程研究室,黑龙江哈尔滨150030

出　　处：《东北农业大学学报》2004年第2期199-204,共6页Journal of Northeast Agricultural University

摘　　要：文章以里氏木霉 (Trichoderma reesei)的基因组 DNA为模板 ,根据 Gen Bank上检索的 β-葡萄糖苷酶基因DNA序列 ,设计特异性引物 ,用高保真酶 probest polymerase进行 PCR扩增 ,获得了 2 .5 0 kb的 DNA片段。将其克隆在 p U C18的 Sma I位点上。测序结果表明 ,所获得的 DNA序列与 Gen Bank上检索的 β-葡萄糖苷酶基因的核苷酸序列同源性达 99.90 % ,氨基酸序列同源性达 10 0 %。On the base of nucleic acid DNA sequence of β-1,4-glucosidase gene a specific primer was synthesized.Using the genomic DNA of Trichoderma reesei as template amplified by PCR with probest polymerase a 2.50 kb DNA fragment was cloned.The fragment was inserted into SmaI site of pUC18.The result of sequence analysis showed that the DNA fragment we got shares 99.99% in base squence with the β-1,4-glucosidase gene in GenbBank and the amino acid sequence shares 100%.

关 键 词：里氏木霉 β—葡萄糖苷酶 基因克隆 序列分析 纤维素 纤维素酶 

分 类 号：Q556.2[生物学—生物化学]