Effects of presenilins and β-amyloid precursor protein on delayed rectifier potassium channels in cultured rat hippocampal neurons  

作　　者：WeiZHANG Hong-weiJIN Xiao-liangWANG 

机构地区：[1]DepartmentofPharmacologyInstituteofMateriaMedica,PekingUnionMedicalCollegeandChineseAcademyofMedicalScience,Beijing100050,China

出　　处：《Acta Pharmacologica Sinica》2004年第2期181-185,共5页中国药理学报（英文版）

基　　金：Project supported by the State Key Technologies R & D Programme (973), No 1998051106.

摘　　要：AIM: To study the effects of presenilin-1 (PS-1), presenilin-2 (PS-2), and amyloid beta-protein precursor (APP695) on delayed rectifier potassium channels (IK) in the cultured rat hippocampal neurons. METHODS: PS-1, PS-2, and APP695 were transfected into the cultured rat hippocampal neurons by transient transfection techniques. The IK current was observed by the whole cell patch-clamp techniques. RESULTS: IK was increased in cultured rat hippocampal neurons, after transient transfection of PS-1, PS-2, and APP695. IK amplitudes and densities were significantly increased from (1689±412) pA, (48±18) pA/pF (mock cells, GFP alone, n=17) to (5565±1403) pA, (252±107) pA/pF (PS-1/GFP, n=22, P<0.01), (3804±1651) pA , (120±58) pA/pF( PS-2/GFP, n=16, P<0.01), and (4978±904) pA, (218±70) pA/pF (APP695, n=22, P<0.01). But PS-1, PS-2, and APP695 did not alter the activa- tion curve of IK (P>0.05). CONCLUSION: Overexpression of PS-1, PS-2, and APP695 increased IK in the cultured rat hippocampal neurons. The upregulation of IK may be related to neuronal apoptosis after PS-1, PS-2, and APP695 were transfected.AIM: To study the effects of presenilin-1 (PS-1), presenilin-2 (PS-2), and amyloid beta-protein precursor (APP695) on delayed rectifier potassium channels (IK) in the cultured rat hippocampal neurons. METHODS: PS-1, PS-2, and APP695 were transfected into the cultured rat hippocampal neurons by transient transfection techniques. The IK current was observed by the whole cell patch-clamp techniques. RESULTS: IK was increased in cultured rat hippocampal neurons, after transient transfection of PS-1, PS-2, and APP695. IK amplitudes and densities were significantly increased from (1689±412) pA, (48±18) pA/pF (mock cells, GFP alone, n=17) to (5565±1403) pA, (252±107) pA/pF (PS-1/GFP, n=22, P<0.01), (3804±1651) pA , (120±58) pA/pF( PS-2/GFP, n=16, P<0.01), and (4978±904) pA, (218±70) pA/pF (APP695, n=22, P<0.01). But PS-1, PS-2, and APP695 did not alter the activa- tion curve of IK (P>0.05). CONCLUSION: Overexpression of PS-1, PS-2, and APP695 increased IK in the cultured rat hippocampal neurons. The upregulation of IK may be related to neuronal apoptosis after PS-1, PS-2, and APP695 were transfected.

关 键 词：PRESENILIN-1 presenilin-2 amyloid beta-protein precursor potassium channels TRANSFECTION patch-  clamp techniques 

分 类 号：R749.16[医药卫生—神经病学与精神病学]