银耳的诱变与营养缺陷型菌株的筛选  

Mutation Inducing of Tremella fuciformis and Screening of Auxotrophic Mutants

作　　者：谢宝贵[1] 朱虎[1] 江玉姬[1] 饶永斌[1] 郑金贵 

机构地区：[1]福建农林大学生命科学学院,福建福州350002

出　　处：《江西农业大学学报》2004年第2期246-249,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：福建省科技基金(2001Z010)资助项目

摘　　要：实验结果表明,银耳酵母状分生孢子对紫外线的效应为多靶单击类型,而对60Coγ射线的效应为单靶单击。紫外线诱变获得2个营养缺陷型菌株,其中1株T811经鉴定是肌醇缺陷型。突变体T811的生长迟缓期比野生型多3d,生长速度较慢。同时还提出了该突变体的应用前景。The results of the experiment indicated that ultraviolet acted as Poly-Targets-and-Sole-Hited effect, and γ ray acted as Sole-Target-and-Sole-Hited effect on Tremella fuciformis. Two mutants were obtained from UV treatment, one of them (T811) was identified as inositol mutant. The mutant T811 grew slower than its wild type Tr21, its Lag-Phase was 3 days longer. The prospect of utilization of the inositol mutants is discussed in this paper.

关 键 词：银耳 诱变 营养缺陷型菌株 筛选 紫外线 Γ射线 生物反应器 分生孢子 突变体 基因工程 

分 类 号：S646.603.6[农业科学—蔬菜学] Q78[农业科学—园艺学]