抗膀胱癌免疫毒素BDI-1-PEA特异杀伤肿瘤细胞的研究  

Specific Killing Effects of Immunotoxin BDI-1-PEA against Human Bladder Cancer Cells

作　　者：温儒民[1] 薛松[1] 孙晓青[1] 陈家存[1] 顾骧[1] 陈仁富[1] 郑骏年[1] 

机构地区：[1]徐州医学院附属医院泌尿外科,江苏徐州221002

出　　处：《中国肿瘤生物治疗杂志》2004年第1期39-41,共3页Chinese Journal of Cancer Biotherapy

基　　金：江苏省教委自然科学基金资助项目(基金编码98KJB320007)

摘　　要：目的:探讨免疫毒素BDI-1-PEA特异杀伤膀胱癌细胞的体外及体内研究。方法:应用MTT法检测不同浓度的BDI—1-PEA,BDI-1和PEA的混合物(BDI—1+PEA),PEA对体外癌细胞的杀伤能力。接种于裸鼠背部皮下的癌细胞长至0.3cm大小实体瘤时,于尾静脉隔日分别注入BDI-1-P=EA、BDI-1、正常小鼠IgG共6次,观察不同的药物对癌的抑制作用。结果:免疫毒素BDI-1—PEA在体外抗膀胱癌细胞系E—J的作用明显优于.PEA及BDI-1与PEA的混合物,在荷瘤裸鼠体内明显优于BDI-1及IgG,与对非靶细胞的细胞毒性作用相比较差异非常显著。结论:免疫毒素BDI-1-PEA是一种很有潜力的抗癌药物,为进一步临床研究奠定基础。Objective: To study the selective killing effects of immunotoxin BDI-1-PEA against human-bladder cancer cell line E-J and nude mice bearing human bladder tumor. Methods; MTT was used to measure the killing ratio of BDI-1-PEA, mixtures of BDI-1 and PEA, PEA against human-bladder cancer cell line E-J at different concentrations in vitro. When the tumor size reached 0. 3 cm, BDI-1-PEA,BDI-1 and IgG were injected respectively to each group of mice though tail vein every other day for 6 times. Determined the inhibitory effects of BDI-1-PEA, BDI-1 and IgG in nude mice bearing human bladder tumor. Results; The results indicated that BDI-1 -PEA showed stronger cytotoxicity against human bladder cancer cell line E-J than PEA and mixtures of BDI-1 and PEA, and stronger cytotoxicity against nude mice bearing human bladder tumor than BDI-1 and IgG. Compared with cytotoxicity against non-target cell, the difference was significant ( P < 0.01). Conclusion: Immunotoxin BDI-1-PEA is a largely potential anticancer drug, the result is a good foundation for the further clinical study.

关 键 词：免疫毒素 膀胱癌 绿脓杆菌外毒素 

分 类 号：R737.14[医药卫生—肿瘤]