大肠杆菌K_(99)遗传工程活疫苗对绵羊的安全性及免疫原性试验研究  

Safety and immunogenicity of genetic engineering live Escherichia coli K99 vaccine for sheep

作　　者：尹德萱 郭海 牛凤兰 常晓琴 郭湘林 阎治川 张治平 马耀岚 刘应江 黄培堂[3] 李丰生[3] 钟声[3] 王叙甫[3] 芮贤良[3] 

机构地区：[1]陕西省榆林地区畜牧兽医研究所,719000 [2]榆林市巴拉素乡兽医站 [3]军事医学科学院生物工程研究所

出　　处：《中国兽医科技》1992年第7期5-7,共3页Chinese Journal of Veterinary Science and Technology

摘　　要：用大肠杆菌K_(99)遗传工程活疫苗,按100亿活菌/只剂量于产前6周给149只妊娠杂交绵羊皮下接种,有28.86％的羊只出现临床反应,但所有免疫母羊均正常分娩,其所产羔羊均发育正常。对免疫母羊所产羔羊(66只)吮吸初乳后,给口服250亿强毒菌攻击,死亡率为4.55％,其发生腹泻持续期平均为1.78天,排菌持续期平均为2.94天。与未免疫对照组比较,差异极显著。本试验初步证明该疫苗对绵羊的安全性与免疫原性良好.When 149 crossbred pregnant sheep had been subcutaneously inoculated with the genetic engineering live E.coli K99 vaccine at dose of 10^(10) live bacteria per sheep, 28.86% of animals inoc-uted showed clinical symptoms, but all these sheep laboured normally and their newborn lambs were developing normally. After the lambs sucked colostrum,they were perorally administrated 2.5×10^(10) virulent live E.coli that 4.55% the lambsdied, the average persistent periods of diarrhea and discharge of bacteria were 1.78 days and 2.94 days, respectively, which differences were obvious in comparison with nor-ino-culated control group.

关 键 词：绵羊 免疫 大肠杆菌 遗传 疫苗 

分 类 号：S858.262.4[农业科学—临床兽医学]