抗真菌多肽捷安肽素发酵条件的研究  

FERMENTATION CONDITIONS FOR ANTIFUNGAL POLYPEPTIDE JIEAN-PEPTIDE

作　　者：赖娅娜[1] 谭红[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041

出　　处：《应用与环境生物学报》2004年第2期231-235,共5页Chinese Journal of Applied and Environmental Biology

基　　金：中国科学院知识创新工程重要方向项目(No.KSCXZ-SW-301-05)~~

摘　　要：对筛选出的一株芽孢杆菌ZK发酵产物抗真菌多肽捷安肽素的发酵培养基组分(碳源、氮源及无机盐)和工艺条件(发酵温度、起始pH,摇床转速,装液量)进行了摸索,通过单因素实验和正交优化实验,确定了ZK菌株发酵培养基最佳组成:生物氮素3.5％、葡萄糖3％、酵母膏0.08％、MgSO4·7H2O 0.5％、KH2PO4·3H2O 0.1％;最适温度为32℃;最适初始pH为8.0.在250 mL三角瓶中装50 mL培养基,于150 r min-1的旋转摇床上32℃振荡培养72 h,ZK菌株产捷安肽素的量达到最大.The fermentation conditions for Bacillus strain ZK to produce a novel polypeptide (Jiean-peptide) were studied and designed by single factor and orthogonal experiments, including temperature, initial pH, nitrogen sources and carbon sources. The optimal fermentation medium was composed of: biology nitrogen 3. 5 % , glucose 3% , yeast extract 0.08% , MgSO4. 7H2O 0. 5% , KH2PO4 ?3H2O 0. 1% and pH 8. 0. When strain ZK was cultured at 30-32℃ for 72 hours on a rotary shaker with a speed of 150 r min-1, it produced the highest amount of Jiean-peptide. Fig 8 , Tab 5 , Ref 14

关 键 词：捷安肽素 抗真菌多肽 发酵 培养基 芽孢杆菌 生物农药 

分 类 号：TQ927[轻工技术与工程—发酵工程] S482.28[农业科学—农药学]