血红素提取纯化新技术研究  

Study on New Technology of Extracting and Sublimating Heme

作　　者：宋照军[1] 刘玺[1] 赵良[1] 张培旗[2] 

机构地区：[1]河南职业技术师范学院,新乡453003 [2]中国海洋大学,青岛266003

出　　处：《广州食品工业科技》2004年第1期6-8,11,共4页

摘　　要：本文对猪血红细胞中血红素的提取工艺进行了研究,通过正交试验确定了提取纯化血红素的最优工艺条件,即:溶血时,添加去离子水1倍,氯仿0.15倍;分离血红素时,加丙酮4倍、同时调节PH值3;结晶血红素时加NaAc1.0%,调节PH值为5。根据本工艺可从1000ml猪血红细胞中提取2500mg、纯度达90%的血红素。This paper researches the technological procedure of heme extracting and sublimating from pig red cells. Through perpendicular testing, we found the excellent procedure condition. during hemolysis, add water 3 times, chloroform 0.25 times, and regulate ph3.0. During extracting, add NaAc 1.0%, regulate ph5.0.By this procedure, we obtained heme 2500mg weight and 90% purity from 1000 ml pig blood.

关 键 词：猪血 血红素 提取工艺 纯化 天然色素 

分 类 号：TS251.9[轻工技术与工程—农产品加工及贮藏工程]