牛磺酸对链脲佐菌素-糖尿病性白内障干预机制初探  

Study on the intervening mechanism of taurine on streptozotocin induced diabetic cataracts

作　　者：宋旭东[1] 陈翠真[2] 董冰[2] 施玉英[1] 张伟[2] 颜流水[3] 罗国安[3] 

机构地区：[1]首都医科大学附属北京同仁眼科中心,100730 [2]北京市眼科研究所 [3]清华大学

出　　处：《中华眼科杂志》2003年第10期605-609,共5页Chinese Journal of Ophthalmology

基　　金：国家自然科学基金资助项目 ( 9770 786 )

摘　　要：目的　探讨不同浓度牛磺酸对链脲佐菌素 (STZ) 糖尿病性白内障的干预机制。方法1 0 0只雄性SD大鼠 ,随机平均分为 5组 ,腹腔注射STZ溶液 (55mg kg体重 )诱发糖尿病性白内障 ,牛磺酸治疗组大鼠每日腹腔注射 1次牛磺酸 (5ml kg体重 )。定期测定各组大鼠血糖浓度 ,实验结束时测定各组血清中甘油三酯等生化指标及胰岛素水平 ,用毛细管电泳仪检测房水及晶状体中牛磺酸含量。结果　STZ组大鼠在诱发的第 3周晶状体开始出现囊泡或轻度混浊 ,而牛磺酸明显抑制了牛磺酸治疗组早期白内障的发生。诱发的第 4～ 1 2周 ,STZ组晶状体混浊度迅速加重 ,而 4 %或 8%牛磺酸组则明显延缓了白内障的发生。 4 %和 8%牛磺酸治疗组在STZ诱发的第 4天、第 4周、第 8周(4%牛磺酸组 )的血糖均显著低于STZ组 ,第 1 2周牛磺酸治疗组与STZ组的血糖值无显著意义。4 %牛磺酸组大鼠血清甘油三酯水平显著低于STZ组 (P =0 0 0 4 ) ,8%牛磺酸组大鼠血清甘油三酯水平也明显低于STZ组 (P =0 0 1 0 )。 8%牛磺酸治疗组大鼠的房水中牛磺酸浓度高于STZ组 ,差异有显著意义 (P =0 0 36) ,其晶状体中牛磺酸浓度也高于STZ组 ,差异有非常显著意义 (P =0 0 0 0 )。结论　牛磺酸对STZ 糖尿病性白内障的干预作用具有剂量依赖性 。Objective To investigate the effects of various concentrations of taurine on streptozotocin (STZ) induced diabetic cataracts in rats by biochemistry, radioimmunology and capillary zone electrophoresis (CZE) Methods One hundred Sprague Dowley (SD) male rats, weight 180～200 g, were randomly divided into 5 groups: normal control, STZ plus saline, STZ plus 2% taurine, STZ plus 4% taurine and STZ plus 8% taurine groups After being fasted for 12 hours, the rats of the STZ group (with or without taurine) were prepared by STZ intraperitoneal injection (55 mg/kg b w ) Taurine treated groups received 2%, 4% and 8% taurine injections once daily for 12 weeks (5 ml/kg b w ) STZ group without taurine received a saline injection once daily for 12 weeks (5 ml/kg b w ) During the experiment period, blood sugar was measured by ONE TOUCH  Ⅱ At the end of experiment, several biochemical parameters were measured by Beckman CX 7, insulin was measured by 125 I insulin radioimmunological detecting kit and the level of taurine in the aqueous humor and the lens was measured by Beckman 5500 capillary zone electrophoresis (CZE) model Results Three weeks after the administration of STZ, the lenses showed vesicle and light opacity in the STZ group Taurine suppressed the occurrence of early stage cataract obviously Four to 12 weeks after the administration of STZ, the lens opacity in the 4% and 8% taurine treatment groups was markedly delayed as compared with that of the STZ group The level of blood glucose in the 4% and 8% taurine groups was decreased evidently compared to that of the STZ group on 4 days, 4 weeks, and 8 weeks (the 4% taurine group) No difference could be detected between the STZ group and taurine treatment groups in 12 weeks In the 4% and 8% taurine groups, the level of triglycerides decreased markedly compared to that of the STZ group and was near that of the control group There were no differences of other biochemical parameters among all groups In the 8% taurine group,

关 键 词：牛磺酸 链脲佐菌素-糖尿病性白内障 治疗 房水 

分 类 号：R776.1[医药卫生—眼科]