畜禽肌肉、内脏及虾中氯霉素残留量的检测技术研究  

Study on the determination of chloramphenicol residues in muscles and viscera of the livestock and poultry and in the shrimp

作　　者：杨大进[1] 蒋定国[1] 王竹天[1] 方从容[1] 文玉雪[2] 王玉莲[3] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100021 [2]吉林省珲春市卫生防疫站 [3]山东省泰安市卫生防疫站

出　　处：《卫生研究》2004年第2期198-201,共4页Journal of Hygiene Research

基　　金：社会公益研究专项资金项目"食物残留物的检测技术与监督技术"

摘　　要：目的　快速、简便、灵敏地监测畜禽肌肉、内脏及虾中的氯霉素残留。方法　建立了高效液相色谱法快速检测技术。动物组织中的氯霉素用乙酸乙酯在超声波振荡下提取 3次 ,提取液经脱水、浓缩 ,再用 0 5mol L高氯酸溶液溶解浓缩瓶中残留物、沉淀蛋白质 ,以正己烷去除脂溶性杂质 ,经 0 4 5 μm滤膜过滤后供HPLC测定含量。 结果　本方法的线性范围为0 1～ 2 0 μg ml,肌肉的最低检出浓度为 1 6 μg kg ,内脏为 3 2 μg kg ,在 2 0～ 10 0 μg kg的加标水平时 ,方法平均回收率为 82 1%～ 96 6 % ,相对标准偏差为 4 5 %～ 9 3%。结论　该方法适用范围广 ,回收率高 ,检测限低 ,简单易行 。Objective A rapid, simple and sensitive high performance liquid chromatographic method for the determination of chloramphenicol residues in muscles and viscera of the livestock and poultry and in the shrimp has been established. Methods\ The chloramphenicol in animal tissues is extracted three times by ethyl acetate in ultrasonic. The extract is dehydrated with Na 2SO 4 and concentrated. The residues are cleaned up by 0 5mol/L HClO 4 and n hexane. The purified solution is detected at UV 278nm by HPLC. Results\ The detection limit of this method is 1 6μg/kg in muscles and 3 2μg/kg in viscera, the recovery is from 82 1% to 96 6%, RSD is 5 3% 6 4%. Conclusion This method can be widely applied to animal foods, and its recovery is high, and its detection limit is low, and it is easily popularized in countryside.

关 键 词：畜禽 氯霉素 虾 兽药残留 

分 类 号：R155.51[医药卫生—营养与食品卫生学] TS207.5[医药卫生—公共卫生与预防医学]