Changes in myocardial collagen content before and after left ventricular assist device application in dilated cardiomyopathy  

作　　者：梁红 Johannes Müller 翁渝国 Gerd Wallukat 付平 林汉生 Sabina Bartel Christoph Knosalla Reinhard Pregla Roland Hetzer 

机构地区：[1]Department of Cardiovascular and Thoracic Surgery, Medical College of Jinan University [2]Deutsches Herzzentrum Berlin [3]Max-Delbrück Centre for Molecular Medicine [4]Department of Cardiovascular Surgery, Institute of Cardiovascular Diseases, Tongji Medical University [5]Department of Medical Statistics, Medical College of Jinan University

出　　处：《Chinese Medical Journal》2004年第3期401-407,共7页中华医学杂志（英文版）

摘　　要：BACKGROUND: The purposes of this study were to confirm the changes in myocardial collagen level after left ventricular assist device (LVAD) support in dilated cardiomyopathy (DCM), find the relation between these changes and prognosis, and test a practical method to assess the level of myocardial collagen. METHODS: Left ventricular samples were collected from DCM patients with different prognosis (transplanted group n = 8, weaning group n = 10) at the time when the LVADs were implanted and again during cardiac transplantation (n = 8). The level of neutral salt soluble collagen (NSC) and acid soluble collagen (ASC) was measured by Sircol collagen assay, and that of total collagen and insoluble collagen (ISC) by quantification of hydroxyproline (Hyp). Serum samples were collected from a portion of these patients (transplanted group, n = 6; weaning group n = 7) at the time the LVADs were implanted, 1 month after implantation and on explantation. Circulating concentration of carboxy-terminal propeptide of type I procollagen (P I CP), amino-terminal propeptide of type I procollagen (P I NP), amino-terminal propeptide of type III procollagen (P III NP) and type I collagen telopeptide (I CTP) were measured by the equilibrium type radioimmunoassay. RESULTS: Before LVAD implantation the level of NSC and ISC in the weaning group was higher but ASC in the transplanted group was lower than in the controls (PBACKGROUND: The purposes of this study were to confirm the changes in myocardial collagen level after left ventricular assist device (LVAD) support in dilated cardiomyopathy (DCM), find the relation between these changes and prognosis, and test a practical method to assess the level of myocardial collagen. METHODS: Left ventricular samples were collected from DCM patients with different prognosis (transplanted group n = 8, weaning group n = 10) at the time when the LVADs were implanted and again during cardiac transplantation (n = 8). The level of neutral salt soluble collagen (NSC) and acid soluble collagen (ASC) was measured by Sircol collagen assay, and that of total collagen and insoluble collagen (ISC) by quantification of hydroxyproline (Hyp). Serum samples were collected from a portion of these patients (transplanted group, n = 6; weaning group n = 7) at the time the LVADs were implanted, 1 month after implantation and on explantation. Circulating concentration of carboxy-terminal propeptide of type I procollagen (P I CP), amino-terminal propeptide of type I procollagen (P I NP), amino-terminal propeptide of type III procollagen (P III NP) and type I collagen telopeptide (I CTP) were measured by the equilibrium type radioimmunoassay. RESULTS: Before LVAD implantation the level of NSC and ISC in the weaning group was higher but ASC in the transplanted group was lower than in the controls (P

关 键 词：Heart-Assist Devices Adult Cardiomyopathy  Dilated Collagen Comparative Study Female Heart Transplantation Humans HYDROXYPROLINE Male Middle Aged MYOCARDIUM PROCOLLAGEN Prognosis 

分 类 号：R542.2[医药卫生—心血管疾病]