阿魏酸对视网膜神经细胞内游离钙浓度变化的影响  

Effect of ferulic acid on the changes of cytosolic free calcium in nerve cells of retina

作　　者：李根林[1] 王津津[1] 刘月月[1] 王景昭[1] 

机构地区：[1]首都医科大学北京同仁眼科中心,北京100730

出　　处：《眼科研究》2004年第1期24-27,共4页Chinese Ophthalmic Research

基　　金：国家"九五"攻关专题计划(96-920-06-05-8);国家新药基金(96-901-05-190);北京市高新技术实验室基金资助

摘　　要：目的研究阿魏酸(FA)对视网膜神经细胞内游离钙([Ca2+]i)水平变化的影响。方法以胚胎7个月人视网膜,新生小牛视网膜和生后4个月小鼠视网膜为研究对象,检测FA作用后[Ca2+]i水平变化。结果500μg/mLFA作用下,胚胎人组[Ca2+]i荧光强度(基值为57.08±3.97)在10s内降至38.41±4.92,维持在38.41±4.92～43.45±3.29;新生小牛组[Ca2+]i(基值为69.48±3.87)在20s内降至52.86±3.69,维持在49.75±3.82～54.39±4.04;成年小鼠组[Ca2+]i(基值为71.54±3.73)在20s内降至54.91±3.57,维持在53.81±3.49～58.14±3.49,且发现胚胎人组[Ca2+]i基值和受光照射后变化明显异于新生期小牛组和成年小鼠组。结论FA能有效降低视网膜神经细胞内游离钙水平,对于促进增殖和保护细胞具有重要作用。Objective To investigate the possible effect of Ferulic acid (FA)on cy tosolic free calcium (i) within nerve cells of retina.Methods In vitro,retina cell suspension fr om7-month human embryo(HE),neonatal calf(NC)and adult mice(AM)were trea ted with50μg /mL FA,and examined using Confocal method to estimate the chan ges of i upon laser illumination.Results i in HE retina was reduced markedly after being treated.In10seconds,it cha nged from57.08±3.97to38.41±4.92,and remained low levels fro m38.41±4.92to43.45±3.29.In20seconds,i in NC retina was changed from69.48±3.87to52.86±3.69,and remained low levels from49.75±3.82to54.39±4.04.i in AM retina was also changed from71.54±3.73to54.91±3.57 in20seconds,and remained low levels between53.81±3.49and58.14 ±3.49.The level and changes of i in HE were also different from that in NC and AM.Conclusion All results ab ove suggest that FA is an effective agent for reducing i in nerve cells of retina,allowing to improve proliferation and protection o f retinal nerve cells.

关 键 词：阿魏酸 视网膜 神经细胞 游离钙 浓度变化 胞浆游离钙 

分 类 号：R774.1[医药卫生—眼科]