大孔离子交换树脂在重组大肠杆菌生产hEGF中的原位分离作用  

IN-SITU SEPARATION BY MACROSPORE-ANIONIC EXCHANGE RESIN DURING RECOMBINANT E.coli CULTIVATION FOR hEGF PRODUCTION

作　　者：陈新爱[1] 徐志南[2] 范立梅[1] 岑沛霖[2] 

机构地区：[1]浙江大学生命科学学院,浙江杭州310029 [2]浙江大学生物工程研究所,浙江杭州310027

出　　处：《磁流体发电情报》2004年第3期493-496,共4页

摘　　要：Production and accumulation of toxic by-products such as acetic acid can inhibit the growth of recombinant cells and the expression of exogenous gene in E.coli. An anionic exchange resin, A-D 3-1, which is high in adsorption selectivity and capability for acetic acid, was screened from a variety of resins based on its physical and chemical properties.On the scale of shake flask culture, the addition of 1.0 g resin per 30 ml medium was insignificant for the cell growth, however,it could improve the hEGF expression significantly. The batch culture in 2.5 L fermentor showed that in-situ adsorption of acetic acid by anionic exchange resin could enhance the expression level of interested protein and reduce the fermentation period by 2 hours. And up to 10% improvement of hEGF (human epidermal growth factor)volumetric productivity (225.0 mg·L -1 ) could be achieved by supplementing 3.3 g resin per 100 ml medium.Production and accumulation of toxic by-products such as acetic acid can inhibit the growth of recombinant cells and the expression of exogenous gene in E.coli. An anionic exchange resin, A-D 3-1, which is high in adsorption selectivity and capability for acetic acid, was screened from a variety of resins based on its physical and chemical properties.On the scale of shake flask culture, the addition of 1.0 g resin per 30 ml medium was insignificant for the cell growth, however,it could improve the hEGF exp...

关 键 词：重组大肠杆菌 HEGF 原位分离 大孔离子交换树脂 

分 类 号：R394.8[医药卫生—医学遗传学]