瘢痕疙瘩Fas基因外显子8突变序列分析  

Detection of LOH in Fas gene deathdoman in keloids using PCR-SSCP and gene sequencing

作　　者：刘永波[1] 高建华[1] 段红杰[1] 刘晓军[1] 

机构地区：[1]第一军医大学南方医院整形科,广东广州510515

出　　处：《中国实用美容整形外科杂志》2004年第2期101-103,共3页

基　　金：国家自然科学基金资助项目 ( 3 9870 80 7)

摘　　要：目的　探讨Fas基因缺失在瘢痕疙瘩形成中的意义。方法　采用PCR -SSCP银染技术及基因序列分析 ,检测 15个瘢痕疙瘩标本Fas基因外显子 8的基因结构。结果　所检测的 15个瘢痕疙瘩标本 10 0 %有Fas基因外显子 8的杂合丢失 ,基因测序发现 11个标本共 4个位点存在基因突变。结论　瘢痕疙瘩Fas基因外显子Objective To detect LOH of Fas gene (exon 8) in fibroblasts of tissues from skin lesions of 15 patients with keloids. Methods The polymerase chain reaction followed by SSCP analysis and direct DNA sequence were used to detect LOH of Fas gene. Results LOH in Fas gene exon 8 were identified in all patients with keloids by PCR-SSCP, Gene sequencing proved these mutations. No Fas gene mutations were found in fibroblasts from healthy skin samples of any of the patients. Conclusion LOH in exon 8 resulted in keloid Fas protein with incomplete deathdoman and closely connected with its nonfunction.

关 键 词：瘢痕疙瘩 Fas基因外显子 基因突变序列 PCR反应 基因测序 

分 类 号：R361[医药卫生—病理学]