胃癌微卫星不稳定性与淋巴结转移的关系  

Relationship Between Microsatellite Instability and Lymph Node Metastasis in Gastric Carcinoma

作　　者：姜斌[1] 翟勇平[1] 刘静[1] 顾军[1] 王雅杰[1] 

机构地区：[1]上海第二军医大学附属长海医院肿瘤科,上海200433

出　　处：《肿瘤防治杂志》2003年第6期604-605,共2页China Journal of Cancer Prevention and Treatment

摘　　要：目的 :探讨胃癌微卫星不稳定性 (microsatelliteinstability ,MSI)与淋巴结转移的关系。方法 :采用聚合酶链反应法 (PCR) ,分别选用定位于第 3及第 17号染色体上的微卫星标记位点 ,对 5 0例原发性胃癌及自身正常组织标本进行检测。结果 :5 0例胃癌中MSI发生率为 4 4% (2 2 / 5 0 ) ,微卫星DNA改变与癌的浸润深度无关 ,P =0 6 5 6 5 ;但有淋巴结转移者明显高于无淋巴结转移者 ,P =0 0 0 4 9。结论 :微卫星DNA的改变不同程度地反映胃癌的细胞生物学恶性行为 ,对胃癌临床分期及预后可能有参考意义。Objective To study the relationship between microsatellite instability(MSI) and lymph node metastasis in gastric carcinoma.Methods Two microsatellite repeatsequences D3S1339(chromosome 3),TP53(chromosome 17) were examined in normal and tumor pairs from 50 gastric carcinoma patients by PCR and denatured polyacrylamide gel electrophoresis.Results The MSI positive was 44%(22/50).The detective rate of alteration of microsatellite had no relation with the depth of invasion( P =0 656 5),but it was significantly higher in cases with lymph node metastasis than that without metastasis( P =0 004 9).Conclusions These results can reflect the cytobiologic malignant behavior of gastric carcinoma in varying degrees,which may be of reference significance in analysing the clinical staging and the prognosis.

关 键 词：胃肿瘤 遗传学 DNA 卫星 聚合酶链反应 

分 类 号：R735.2[医药卫生—肿瘤]