端粒酶基因在脑胶质瘤中的原位表达  

Expression of Telomerase (hTR) in Situ in Brain Glioma

作　　者：王东林[1] 施公胜[2] 鄂群[1] 黄华[2] 

机构地区：[1]南通医学院病理学教研室,江苏南通226001 [2]南通医学院附属医院病理科,江苏南通226001

出　　处：《肿瘤防治杂志》2003年第10期1036-1039,共4页China Journal of Cancer Prevention and Treatment

基　　金：江苏省教育厅资助项目 (批准号 0 1KJD 3 10 0 0 8)

摘　　要：目的 :研究端粒酶基因 (hTR)在脑胶质瘤中的原位表达状况及其与分型、分级的关系 ,评估其对胶质瘤诊断的价值。方法 :用原位杂交技术检测了 79例甲醛固定、石蜡包埋的胶质瘤蜡块标本中端粒酶基因 (hTR)的表达状况并分析与组织学分级、WHO分型之间的关系。用ABC法检测PCNA的表达。 6例正常脑组织作对照。结果 :端粒酶基因 (hTR)在脑胶质瘤中的检出率为 5 9 2 % (4 7/ 79)。端粒酶基因 (hTR)在胶质瘤组织学分级中的分布为Ⅱ级 11/ 32 ,Ⅲ级 12 / 2 0 ,Ⅳ级 2 4 / 2 7,各级胶质瘤组间比较 ,差异有显著意义 ,P <0 0 5。端粒酶基因 (hTR)的表达强度与胶质瘤的组织学分级、WHO分型之间有相关性 ,P <0 0 5 ,Ⅲ级、Ⅳ级的检出率明显高于Ⅱ级。正常脑组织中未检出端粒酶基因。端粒酶阳性组与阴性组PCNA阳性细胞密度差异有显著意义 ,P <0 0 5。结论 :胶质瘤中端粒酶基因 (hTR)表达状况可能与胶质瘤的分化程度、组织学分级相关 ,提示端粒酶基因的过表达可能对胶质瘤的演化和进展具有一定重要作用 ,并可能作为胶质瘤的一个新的诊断标志物 ,结合PCNA评价胶质瘤的生物学行为更为可靠。Objectives To study the expression of telomerase (hTR) in situ in glioma,and the possible relationship between hTR expression and typing and grading in glioma,and to determine its value in clinical diagnosis of glioma.Methods Telomerase(hTR)expression was detected in 79 cases of formalin fixed,paraffin embeded glioma by in situ hybridization (ISH),and its relationship to histological grade,WHO typing was analysed.PCNA expression was detected by ABC.Six cases of normal brain were used as control.Results hTR positive rate in 79 cases of glioma was 59 2%(47/79),the distribution of expression of telomerase hTR was 11/32 in grade Ⅱ glioma,12/20 in grade Ⅲ,24/27 in grade Ⅳ,respectively.The difference among every group was significant, P <0 05.Expressive intensity of telomerase (hTR) was associated with their histological grade and WHO typing, P <0 05.The positive rates of grade Ⅲ/Ⅳ were higher than that of grade Ⅱ,while normal brain tissue was negative.Positive cell dense of PCNA between positive and negative groups of hTR had a significant difference.Conclusion The expression of telomerase (hTR)may be associated with differenciation and histological grading.It is suggested that excessive telomerase(hTR) expression play an important role in the development and progression of the tumor,and thus may become a new diagnostic marker of glioma.Combining PCNA is more reliable to evaluate biological characters of glioma.

关 键 词：脑肿瘤 酶学 脑肿瘤 诊断 神经胶质瘤 酶学 神经胶质瘤 诊断 端粒 末端转移酶 代谢 

分 类 号：R739.4[医药卫生—肿瘤]