气相色谱-质谱选择离子法检测精神分裂症呼出气体中的戊烷、异戊二烯和二硫化碳  

Determination of Isoprene,Pentane and Carbon Disulifide in Breath of Schizophrenia by Selected Ion Monitoring Gas Chromatography-mass Spectrometry

作　　者：张泉水[1] 林勇强 林凌云[1] 庄希航[1] 陈平周[1] 赵虎[3] 戚元丽 

机构地区：[1]汕头大学精神卫生中心,广东汕头515063 [2]广东省精神卫生研究所,广东广州510180 [3]汕头大学医学院法医病理学教研室,广东汕头515041

出　　处：《汕头大学医学院学报》2003年第4期195-197,共3页Journal of Shantou University Medical College

基　　金：广东省医学科研基金 (A2 0 0 2 460 )

摘　　要：目的 :检测呼出气体中的戊烷、异戊二烯和二硫化碳 ,为精神分裂症进行呼吸试验研究提供方法学依据。方法 :直接呼气到容积为 2L的气体采样袋 ,用一次性注射器进样 5mL到气相色谱 ,利用四极杆质谱作为检测器 ,使用选择性离子扫描的方法来进行定性和定量分析。结果 :精神分裂症组呼出气体中的异戊二烯高于情感性精神障碍组和正常对照组 ,但是并没有检测出戊烷和二硫化碳。 2 4个试验对象都能够接受本试验 ,而且没有任何不适。结论 :该呼吸试验操作方便 ,易于接受 。Objective:To describe a method for the determination of pentane,isoprene and carbon disulifide in human breath,which was useful in study of schizophrenia.Methods:The sample of breath was collected by breathing into a two-litre gas sampling bag,then 5mL of sample was assayed by selected ion monitoring gas chromatogrph with quadrupole mass analyzer.Results:Isoprene was significantly higher in the breath of schizophrenias than that in the control groups.Samples of breath were obtained from all subjects without any adverse effects.Conclusion:The method is convenient to operate and is well tolerated by subjects,however,this method is limited when used to detect some substances whose concentrations are more low in breath.

关 键 词：气相色谱-质谱 离子法检测 精神分裂症 呼出气体 戊烷 异戊二烯 二硫化碳 

分 类 号：R749.3[医药卫生—神经病学与精神病学] O657.71[医药卫生—临床医学]