转mtlD/gutD双价基因稻米对大鼠的毒性试验  

A Toxicity Test of Transgenic Rice with mtlD/gutD Gene On Rats

作　　者：王慧中[1] 应奇才[2] 钟国庆[2] 田菊霞[2] 陈河[2] 黄大年[3] 钱前[3] 张红生[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095 [2]杭州师范学院生化与分子生物学重点实验室 [3]中国水稻研究所,浙江杭州310006

出　　处：《中国水稻科学》2004年第3期208-212,共5页Chinese Journal of Rice Science

基　　金：国家863计划资助项目(JY031310);国家自然科学基金资助项目(30370132);浙江省自然科学基金资助项目(302061);中国水稻科学发展基金资助项目(0003239);浙江省科技计划项目(011102186)

摘　　要：对转mtlD/ gutD 双价基因稻米进行了大鼠急性、亚急性毒性实验 ,致突变试验和 30d喂养试验。结果表明 ,大鼠经口LD50 >30g/kg体质量 ,无致突变作用 ;30d喂养试验中 ,18、36、5 4g/kg体质量各剂量组大鼠发育、增重、食物利用率、血常规、脏体比及病理组织学观察等各项指标与基础对照组相比均无显著差异。初步表明转mtlD/ gutD 双价基因稻米对大鼠来说是安全的。Toxicity experiments, mutation experiments and 30-day feeding tests were conducted on rats with transgenic rice bearing mtlD/gutD gene. Results showed that oral LD 50 for rats was larger than 30 g/kg and no mutations were found. The rats groups with 18,36,54 g/kg-dosage had good development in 30-day feeding test. No obvious differences were found in the index of weight, food utilization rate, routine blood test, rate of organ to body (weight to weight), and patho-histological observations between the rats fed with or without the transgenic rice grain. It could be concluded that transgenic rice with mtlD/gutD gene was safe to the rats.

关 键 词：转基因食品 稻米 毒性试验 致突变试验 喂养试验 大鼠 

分 类 号：S188[农业科学—农业基础科学]