Differential expression of genes during aflatoxin B_1-induced hepatocarcinogenesis in tree shrews  

作　　者：YuanLi Da-FangWan Jian-JiaSu Jicao ChaoOu Xiao-KunQiu Ke-ChenBan ChunYang Liu-LiangQin DanLuo Hui-FenYue Li-ShengZhang Jian-RenGu 

机构地区：[1]DepartmentofExperimentalPathology,GuangxiCancerInstitute,Nanning53002l,GuangxiZhuangAutonomousRegion,China [2]NationalLaboratoryforOncogeneandRelatedGenes,ShanghaiCancerInstitute,Shanghai200032,China [3]DepartmentofMolecularBiology,GuangxiCancerInstitute,Nanning530021,GuangxiZhuangAutonomousRegion,China

出　　处：《World Journal of Gastroenterology》2004年第4期497-504,共8页世界胃肠病学杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China,NO.39860072 and NO.39869001;Natural Science Foundation of Guangxi Zhuang Autonomous Region,NO.9817137

摘　　要：AIM:Through exploring the regulation of gene expression during hepatocarcinogenesis induced by aflatoxin B1 (AFB1),to find out the responsible genes for hepatocellular carcinoma (HCC) and to further understand the underlying molecular mechanism.METHODS:Tree shrews ( Tupaia belangeri chinensis)were treated with or without AFB1 for about 90 weeks. Liver biopsies were performed regularly during the animal experiment. Eight shares of total RNA were respectively isolated from 2 HCC tissues, 2 HCC-surrounding noncancerous liver tissues, 2 biopsied tissues at the early stage(30^th week) of the experiment from the same animals as above, 1 mixed sample of three liver tissues biopsied at the beginning (0^th week) of the experiment, and another i mixed sample of two liver tissues from the untreated control animals biopsied at the 90^th week of the experiment. The samples were then tested with the method of Atlas^TM cDNA microarray assay. The levels of gene expression in these tissues taken at different time points during hepatocarcinogenesis were compared.RESULTS:The profiles of differently expressed genes were quite different in different ways of comparison.At the same period of hepatocarcinogenesis, the genes in the same function group usually had the same tendency for up-or down-regulation. Among the checked 588 genes that were known to be related to human cancer, 89 genes (15.1%) were recognized as “important genes” because they showed frequent changes in different ways of comparison. The differentially expressed genes during hepatocarcinogenesis could be classified into four categories: genes up-regulated in HCC tissue, genes with similar expressing levels in both HCC and HCC-surrounding liver tissues which were higher than that in the tissues prior to the development of HCC,genes down-regulated in HCC tissue, and genes up-regulated prior to the development of HCC but down-regulated after the development of HCC.CONCLUSION: A considerable number of genes could change their expressing levels both in HCC and in HCC-suAIM:Through exploring the regulation of gene expression during hepatocarcinogenesis induced by aflatoxin B_1 (AFB_1), to find out the responsible genes for hepatocellular carcinoma (HCC) and to further understand the underlying molecular mechanism. METHODS:Tree shrews (Tupaia belangerichinensis)were treated with or without AFB_1 for about 90 weeks.Liver biopsies were performed regularly during the animal experiment.Eight shares of total RNA were respectively isolated from 2 HCC tissues,2 HCC-surrounding non- cancerous liver tissues,2 biopsied tissues at the early stage (30th week) of the experiment from the same animals as above,1 mixed sample of three liver tissues biopsied at the beginning (0th week) of the experiment,and another 1 mixed sample of two liver tissues from the untreated control animals biopsied at the 90th week of the experiment.The samples were then tested with the method of Atlas^(TM) cDNA microarray assay.The levels of gene expression in these tissues taken at different time points during hepatocarcinogenesis were compared. RESULTS:The profiles of differently expressed genes were quite different in different ways of comparison.At the same period of hepatocarcinogenesis,the genes in the same function group usually had the same tendency for up-or down-regulation.Among the checked 588 genes that were known to be related to human cancer,89 genes (15.1%) were recognized as“important genes”because they showed frequent changes in different ways of comparison.The differentially expressed genes during hepatocarcinogenesis could be classified into four categories:genes up-regulated in HCC tissue,genes with similar expressing levels in both HCC and HCC-surrounding liver tissues which were higher than that in the tissues prior to the development of HCC, genes down-regulated in HCC tissue,and genes up-regulated prior to the development of HCC but down-regulated after the development of HCC. CONCLUSION:A considerable number of genes could change their expressing levels both in HCC and in HCC- surrounding

关 键 词：肝细胞癌 肿瘤生成 分子机制 黄曲霉毒素 肿瘤转移 肿瘤病理学 

分 类 号：R735.7[医药卫生—肿瘤]