肝癌组织中PTEN基因蛋白的表达及其与微血管密度的相关性  

Relationship between PTEN expression and microvessel density in hepatocellular carcinoma

作　　者：王丽[1] 王文亮[2] 郭双平[1] 张勇[2] 成胜权[2] 

机构地区：[1]第四军医大学西京医院病理科,陕西西安710033 [2]第四军医大学基础部病理学教研室,陕西西安710033

出　　处：《第四军医大学学报》2004年第5期402-405,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金 (30 0 0 0 0 76)

摘　　要：目的 :研究原发性肝癌 (HCC)组织中PTEN蛋白的表达及与肿瘤微血管密度 (MVD)之间的相关性 .方法 :应用免疫组织化学SP法检测 5 6例原发性肝癌组织及 4例肝硬化和 1例正常肝组织中PTEN蛋白的表达并对肿瘤MVD计数 ,分析其意义及两者间的相关性 .结果 :肝癌组织中PTEN阳性染色主要定位于细胞核中 ,总阳性率为 4 2 .9% (2 4 / 5 6 ) ;癌旁组织中PTEN阳性染色主要定位于细胞质 ,阳性率为1 0 0 % (32 / 32 ) ,在肝癌组织中的阳性表达率明显低于癌旁组织 (P <0 .0 1 ) ,且其表达强度在HCC病理分级中有差异 ,同时 ,在肿瘤TNM分期中各期PTEN阳性率有显著差异 ,而发生肝内转移和肿瘤直径大于 5cm的肝癌组织PTEN阳性率低于未转移和肿瘤直径小于 5cm的肝癌组织 .肝癌组织中MVD为 4 0± 1 1 ,MVD值随着肿瘤TNM分期的增高而增大 .且MVD在无包膜肝癌中及有肝内转移肝癌中比有包膜和无肝内转移的高 .肝癌组织中PTEN的表达与肿瘤MVD相关性分析两者呈显著负相关 ,即高PTEN表达的HCC中微血管计数较低 ,而低PTEN表达的HCC微血管计数增高 .结论 :PTEN蛋白的低表达与血管生成是原发性肝癌发生发展过程中的重要事件 ,且与肿瘤恶性分化程度密切相关 ;AIM: To study the relationship between PTEN expression and microvessel density (MVD) in hepatocelluar carcinoma (HCC). METHODS: Expressions of PTEN protein and MVD in 56 cases of HCC, 4 cases of hepatocirrhosis and 1 case of normal liver were examined by immunohisochemical SP method. RESULTS: The PTEN positive staining was mainly found in the nuclear. The total positive rate was 42.9% (24/56) and was lower than that in paracarcinoma, which was 100%. The positive rates of PTEN decreased with the progression of pathological grade. And so was the TNM class. We also found that the positive rate of PTEN with intrahepatic recurrence was lower. Of all the HCC, the average MVD was 40±11 and the microvessel counting in HCC was significantly correlated with the tumor capsule formation, intrahepatic recurrence and TNM class. An inverse correlation was found between the PTEN expression and MVD in HCC. CONCLUSION: The low PTEN expression and angiogenesis play important roles in HCC and are closely correlated with the differential grade of HCC. Loss of PTEN protein expression may play a certain role in the angiogenesis in HCC.

关 键 词：肝细胞癌 PTEN基因 抑癌基因 免疫组织化学 微血管密度 

分 类 号：R735.7[医药卫生—肿瘤]