A POTENTIAL TUMOR SUPPRESSOR GENE: Doc-1R  

作　　者：生秀杰 周伟强 姜莉 张梅英 王太一 张学 

机构地区：[1]Department of Laboratory Animal, China Medical University [2]Key Laboratory of Cell Biology, Ministry of Public Health of P.R. China, China Medical University

出　　处：《Chinese Journal of Cancer Research》2004年第1期15-19,共5页中国癌症研究（英文版）

基　　金：Thisworkwassupportedbythenational9thFive-YearplanResearchProgramofChina(No.96-A230602)andtheNationalNaturalScienceFoundationofChina(No.39980011).

摘　　要：Objective: To detect the expression and the genomic sequence of Doc-1R gene in mice. Methods: The gene specific primers were designed and synthesized according to the cDNA sequence of Doc-1R gene. The sequence of Doc-1R gene was cloned by nested PCR. The expression of Doc-1R gene was examined by RT-PCR in thirteen kinds of tissues of mice. Results: The mouse Doc-1R gene has been obtained by two times genomic walking, which spans 2787 bp and contains four exons and three introns. All of the splice donor/acceptor site sequences are in accordance with the consensus 揋T-AG?rule. There was expression of Doc-1R gene in the thirteen tissues. Conclusion: The mouse Doc-1R gene was cloned successfully. The expression pattern suggests that Doc-1R gene is a housekeeping gene, which is important to keep the function of tissues and organs.Objective: To detect the expression and the genomic sequence of Doc-1R gene in mice. Methods: The gene specific primers were designed and synthesized according to the cDNA sequence of Doc-1R gene. The sequence of Doc-1R gene was cloned by nested PCR. The expression of Doc-1R gene was examined by RT-PCR in thirteen kinds of tissues of mice. Results: The mouse Doc-1R gene has been obtained by two times genomic walking, which spans 2787 bp and contains four exons and three introns. All of the splice donor/acceptor site sequences are in accordance with the consensus 揋T-AG?rule. There was expression of Doc-1R gene in the thirteen tissues. Conclusion: The mouse Doc-1R gene was cloned successfully. The expression pattern suggests that Doc-1R gene is a housekeeping gene, which is important to keep the function of tissues and organs.

关 键 词：DOC-1R DNA sequencing Gene expression 

分 类 号：R73-3[医药卫生—肿瘤]