卟啉锰-TFO-吖啶的合成  

Synthesis of manganese porphyrin-TFO-acridine

作　　者：光丽霞[1] 袁发焕[2] 姜中兴[3] 王升启[4] 赵聪敏[1] 刘立[1] 温恩懿[1] 奚敏[1] 艾友萍[1] 管伟[4] 

机构地区：[1]第三军医大学附属新桥医院儿科,重庆400037 [2]第三军医大学附属新桥医院肾内科,重庆400037 [3]武汉大学化学系,武汉430072 [4]军事医学科学院放射医学研究所,北京100039

出　　处：《第三军医大学学报》2003年第14期1258-1261,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 3980 0 12 8)

摘　　要：目的　合成卟啉锰 TFO 吖啶化合物。方法　合成含 7 去氮 2 脱氧黄嘌呤的TFO ;以吖啶修饰TFO的 3′末端 ,以 6碳氨基连接臂修饰TFO的 5′末端 ;四苯基卟啉四羧酸经金属化、酯化后 ,与TFO的 5′末端偶联 ;薄层层析纯化 ,质谱、紫外光光谱分析鉴定。结果　薄层层析结果显示在对照寡核苷酸带前方有一条淡黄色的吖啶 TFO带 ;紫外光光谱分析显示卟啉锰 TFO 吖啶化合物同时具有 2 60nm处寡核苷酸的特征吸收峰和 468nm处卟啉锰的特征吸收峰。质谱分析结果证实 ,卟啉锰 TFO 吖啶化合物分子量实测值与理论值一致。结论　合成了卟啉锰 TFOObjective To synthesize manganese porphyrin triple helix forming oligonucleotide acridine compound. Methods TFO containing 7 deaza 2′ deoxyxanthosine was synthesized with special oxidizing agent. Acridine and amino modifier C6 were bound to the 3′ end and the 5′ end of the oligonucleotide, respectively. After metallation and esterification of meso tetrakis(4 carbocyphenyl), it was bound to the 5′ end of the previously described oligonucleotide. The reaction product was separated and purified by thin layer chromatography(TLC), and then identified by mass spectrography and ultraviolet absorption spectrometry. Results Thin layer chromatography showed that there was a yellow acridine oligonucleotide band before the control oligonucleotide. Ultraviolet absorption spectrum analysis revealed that the manganese porphyrin triple helix forming oligonucleotide acridine compound possessed both characteristic absorption peaks of oligonucleotide at wavelength 260 nm and manganese porphyrin at wavelength 468 nm. Mass spectrogram confirmed that the practically detected molecular weight of the observed mass of TFO derivative was the same as the theoretical value. Conclusion Manganese porphyrin triple helix forming oligonucleotide acridine compound is synthesized successfully.

关 键 词：卟啉锰-TFO-吖啶 合成 三股螺旋形成寡核苷酸 基因治疗 

分 类 号：Q524[生物学—生物化学] R914.5[医药卫生—药物化学]