鼠疫耶尔森菌DNA标识序列的鉴定及其应用研究  

Detection of DNA tag sequences for rapid identification of Yersinia pestis

作　　者：韩延平[1] 周冬生[1] 宋亚军[1] 裴德翠[1] 李敏[2] 崔百忠[2] 包静月[3] 张秀清[3] 童宗中[3] 王津[1] 郭兆彪[1] 祁芝珍[2] 金丽霞[2] 翟俊辉[1] 杜宗敏[1] 王效义[1] 汪建[3] 黄培堂[1] 杨瑞馥[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071 [2]青海省地方病预防控制所 [3]中国科学院北京基因组研究所

出　　处：《解放军医学杂志》2004年第4期307-309,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家"8 63"基金 (编号 2 0 0 1AA2 2 30 61 );国家自然科学基金 (编号30 371 2 84)资助课题

摘　　要：目的　确定鼠疫耶尔森菌DNA标识序列 ,以用于鼠疫耶尔森菌的快速检测和鉴定。方法　通过芯片比较基因组杂交和PCR验证 ,鉴定鼠疫耶尔森菌DNA标识序列 ,针对标识序列设计特定的PCR引物。结果　鼠疫耶尔森菌基因组中存在 3个区段 ,共 2 8条基因 ,均是鼠疫耶尔森菌DNA标识序列。针对其中 3条基因设计PCR引物 ,能特异性扩增出鼠疫耶尔森菌 ,与来自其他非鼠疫耶尔森菌的DNA无交叉反应。结论　鼠疫耶尔森菌存在DNA标识序列 ,并能用于鼠疫耶尔森菌的快速检测与鉴定。Objective To identify the DNA tag sequences with the purpose of rapid and specific characterization of Y. pestis. Methods DNA microarray hybridization combined with PCR was used to perform genomic comparison between strains of Y. pestis and Y. pseudotuberculosis in order to screen and identify Y. pestis-specific genes. Results Twenty eight signature genes of Y. pestis were discovered. Three pairs of Y. pestis-specific primers were designed according to tag genes and proved to amplify the specific sequences of the target bacterium, showing no cross-reaction with the closely related Y. pseudotuberculosis and a large collection of genomic DNAs from other organisms. Conclusion DNA tag sequence is an ideal target for the rapid detection and identification of Y. pestis by PCR method.

关 键 词：耶尔森氏菌 鼠疫 DNA芯片 标识序列 

分 类 号：R378.61[医药卫生—病原生物学]