鼠疫耶尔森菌活疫苗株的比较基因组学研究  

DNA microarray-based comparative genomic analysis of live plague vaccines

作　　者：周冬生[1] 韩延平[1] 戴二黑[1] 王津[1] 宋亚军[1] 童宗中[2] 裴德翠[1] 张玲[1] 包静月[2] 李敏[3] 崔百忠[3] 张秀清[2] 郭兆彪[1] 祁芝珍[3] 金丽霞[3] 翟俊辉[1] 杜宗敏[1] 王效义[1] 汪建[2] 黄培堂[1] 杨瑞馥[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071 [2]中国科学院北京基因组研究所 [3]青海省地方病预防控制所

出　　处：《解放军医学杂志》2004年第4期310-314,共5页Medical Journal of Chinese People's Liberation Army

基　　金：国家"8 63"基金 (编号 2 0 0 1AA2 2 30 61 );国家自然科学基金 (编号30 371 2 84)资助课题

摘　　要：目的　揭示不同来源的鼠疫活疫苗株在基因组组成上的差异。方法　以芯片比较基因组杂交为主要研究手段 ,结合PCR验证 ,对 19株鼠疫活疫苗株进行比较基因组分析。结果　鼠疫活疫苗株基因组中缺失了大量基因 ,但也有某些大片段基因的拷贝增多。结论　不同来源的疫苗株在基因组组成上存在差异 ,构成了不同疫苗株的表型与使用效果具有差异性的遗传基础。Objective To identify and compare the genome differences among live plague vaccines prepared with different strains of the bacillus. Methods The whole-genome DNA microarray of Yersinia pestis was used as a tool to perform genomic comparison among live plague vaccines prepared with 19 different strains. Results Dozens of deletions and/or increased copies of the genomic fragments were identified in the studied vaccines of different strains. Conclusion The revealed genomic differences among the vaccines from different origins account for the variability of the immunogenic and protective potency of live plague vaccines. The whole-genome DNA microarray was also provesd to be an ideal tool for the pre-evaluation of a vaccine strain.

关 键 词：耶尔森氏菌 鼠疫 鼠疫活疫苗 DNA芯片 比较基因组学 

分 类 号：R378.61[医药卫生—病原生物学]