外环流气升式反应器发酵生产聚β-羟基丁酸酯  

Studies on producing poly-β-hydroxybutyrate in external loop airlift reactor

作　　者：黄建新[1] 秦华明[1] 杨金水[1] 唐雪玲[1] 赵景联[2] 

机构地区：[1]西北大学生命科学学院,陕西西安710069 [2]西安交通大学环境与化学工程学院,陕西西安710049

出　　处：《西北大学学报（自然科学版）》2004年第2期199-202,共4页Journal of Northwest University（Natural Science Edition）

基　　金：国家"863"计划新材料领域资助项目(715 004 0160)

摘　　要：目的　提高Z5 G菌株发酵生产聚β 羟基丁酸酯(PHB)产量。方法　采用改变营养配比、通气条件,研究了Z5 G菌在3L外环流气升式反应器中的最佳发酵工艺条件。结果　表明该菌株在C源N源配比为4∶1,发酵前期通气量为1L/L·min-1,后期为0 7L/L·min-1,发酵时间为36h,PHB发酵产率可提高10 7%。结论　Z5 G菌株在外环流反应器中发酵PHB比机械搅拌反应器产率高,发酵周期短,从而降低了能耗,对工业化发酵生产PHB具有实用意义。AimIn order to improve the fermentation production of PHB by the strain Z_5-G.MethodsThe proportion of carbon to nitrogen and the quantity of air ventilation is changed to study the optimum process of fermentation production of PHB,produced by the strain Z_5-G in ELALR of 3L volume.ResultWhen the proportion of carbon to nitrogen is 4∶1, the quantity of air ventilation in the former period is 1 L/L·min^(-1),in the later period that is 0.7 L/L·min^(-1) and the period of fermentation is 36 hours,the fermentation productivity can be increased by 10.7%.ConclusionIn ELALR the strain Z_5-G shows a typical growth regularity of microorganisms.The process can not only reduce the energy consumption and make the operation more convenient but also shorten the period of fermentation than in the mechanical agitated fermentor and increase the fermentation productivity. So the process is applicable for the industrial fermentation production of PHB.

关 键 词：聚Β-羟基丁酸酯 PHB 发酵 生物量 外环流气升式反应器 ELALR 

分 类 号：TS264.4[轻工技术与工程—发酵工程]