N-acetylcysteine attenuates alcohol-induced oxidative stress in the rat  

作　　者：Resat Ozaras Veysel Tahan Seval Aydin Hafize Uzun Safiye Kaya Hakan Senturk 

机构地区：[1]Department of Infectious Diseases and Clinical Microbiology,University of Istanbul,Istanbul,Turkey [2]Department of Internal Medicine,University of Istanbul,Istanbul,Turkey [3]Department of Biochemistry,University of Istanbul,Istanbul,Turkey

出　　处：《World Journal of Gastroenterology》2003年第1期125-128,共4页世界胃肠病学杂志（英文版）

基　　金：the research Fund of the University of Istanbul.No: T-589/240698

摘　　要：AIM: There is increasing evidence that alcohol-induced liverdamage may be associated with increased oxidative stress.We aimed to investigate free-radical scavenger effect of n-acetylcysteine in rats intragastrically fed with ethanol.METHODS: Twenty-four rats divided into three groups werefed with ethanol (6 g/kg/day, Group 1), ethanol and n-acetylcysteine (1 g/kg, Group 2), or isocaloric dextrose(control group, Group 3) for 4 weeks. Then animals weresacrificed under ether anesthesia, intracardiac blood andliver tissues were obtained. Measurements were performedboth in serum and in homogenized liver tissues.Malondialdehyde (MDA) level was measured by TBARSmethod. Glutathione peroxidase (GSH-Px) and superoxidedismutase (SOD) levels were studied by commercial kits.Kruskal-Wallis test was used for statistical analysis.RESULTS: ALT and AST in Group 1 (154 U/Land 302 U/L,respectively) were higher than those in Group 2 (94 U/L and155 U/L) and Group 3 (99 U/L and 168 U/L) (P＝0.001 forboth). Serum and tissue levels of MDA in Group 1 (1.84 nmol/mL and 96 nmol/100 mg-protein) were higher than Group 2(0.91 nmol/mL and 64 nmol/100 mg-protein) and Group 3(0.94 nmol/mL and 49 nmol/100 mg-protein) (P＜0.001 forboth). On the other hand, serum GSH-Px level in Group 1(8.21 U/g-Hb) was lower than Group 2 (16 U/g-Hb) andGroup 3 (16 U/g-Hb) (P＜0.001). Serum and liver tissue levelsof SOD in Group 1 (11 U/mL and 26 U/100 mg-protein)were lower than Group 2 (18 U/mL and 60 U/100 mg-protein)and Group 3 (20 U/mL and 60 U/100 mg-protein) (P＜0.001for both).CONCLUSION: This study demonstrated that ethanol-induced liver damage is associated with oxidative stress,and co-administration of n-acetylcysteine attenuates thisdamage effectively in rat model.AIM:There is increasing evidence that alcohol-induced liver damage may be associated with increased oxidative stress. We aimed to investigate free-radical scavenger effect of n- acetylcysteine in rats intragastrically fed with ethanol. METHODS:Twenty-four rats divided into three groups were fed with ethanol(6 g/kg/day,Group 1),ethanol and n- acetylcysteine(1g/kg,Group 2),or isocaloric dextrose (control group,Group 3)for 4 weeks.Then animals were sacrificed under ether anesthesia,intracardiac blood and liver tissues were obtained.Measurements were performed both in serum and in homogenized liver tissues. Malondialdehyde(MDA)level was measured by TBARS method.Glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)levels were studied by commercial kits. Kruskal-Wallis test was used for statistical analysis. RESULTS:ALT and AST in Group 1(154 U/L and 302 U/L, respectively)were higher than those in Group 2(94 U/L and 155 U/L)and Group 3(99 U/L and 168 U/L)(P=0.001 for both).Serum and tissue levels of MDA in Group 1(1.84 nmol/ mL and 96 nmol/100 mg-protein)were higher than Group 2 (0.91 nmol/mL and 64 nmol/100 mg-protein)and Group 3 (0.94 nmol/mL and 49 nmol/100 mg-protein)(P<0.001 for both).On the other hand,serum GSH-Px level in Group 1 (8.21 U/g-Hb)was lower than Group 2(16 U/g-Hb)and Group 3(16 U/g-Hb)(P<0.001).Serum and liver tissue levels of SOD in Group 1(11 U/mL and 26 U/100 mg-protein) were lower than Group 2(18 U/mL and 60 U/100 mg-protein) and Group 3(20 U/mL and 60 U/100 mg-protein)(P<0.001 for both). CONCLUSION:This study demonstrated that ethanol- induced liver damage is associated with oxidative stress, and co-administration of n-acetylcysteine attenuates this damage effectively in rat model.

关 键 词：Oxidative Stress ACETYLCYSTEINE Animals Erythrocytes Ethanol dosage Free Radical Scavengers GLUTATHIONE Glutathione Peroxidase Lipid Peroxidation Liver Male RATS Rats  Wistar Research Support  Non-U.S. Gov't Superoxide Dismutase Thiobarbituric Acid Reactive Substances 

分 类 号：R575[医药卫生—消化系统]