高脂高蔗糖饲料诱导贵州小香猪发生糖尿病和动脉粥样硬化病变  

Diet of High-Fat and High-Sucrose Induced Diabetes and Atherosclerosis in Minipig

作　　者：席守民[1] 廉馨[2] 王宗保[1] 张秋菊[1] 唐朝克[2] 王佐[2] 尹卫东[1] 

机构地区：[1]南华大学生物化学与分子生物学教研室,湖南省衡阳市421001 [2]南华大学心血管病研究所,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2004年第1期5-10,共6页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金 ( 3 0 3 70 675 );湖南省教育厅课题(JY 0 1C193 )资助

摘　　要：用贵州小香猪建立一种新的 2型糖尿病合并动脉粥样硬化模型 ,探讨高脂高蔗糖饲料对糖、脂代谢的影响。采用高脂高蔗糖饲料饲喂贵州小香猪 ,观察其体重、血糖、胰岛素和血脂的变化 ,并对胰岛 β细胞功能、脂质在主动脉和肝脏组织沉积进行研究。结果发现 ,喂养 8个月后 ,与正常对照组比较 :实验组空腹血糖值明显升高 ,血清总胆固醇、甘油三酯水平升高 ,脂肪细胞肥大 ,胰岛 β细胞受损 ,主动脉出现脂纹 ,内膜增厚 ,肝脂肪样变性。以上结果说明 ,高脂高蔗糖饲料可诱导贵州小香猪发生胰岛素抵抗、糖尿病和动脉粥样硬化 ,此模型适用于糖尿病并发脂代谢紊乱和动脉粥样硬化及其治疗的研究。Aim To establish a new animal model of the human type 2 diabetes mellitus complicated with atherosclerosis and investigate the effects of high fat/high sucrose feeding on glucose and lipid metabolism in guizhou minipigs. Methods Minipigs were fed with high fat/high sucrose diet (HFSD) and the levels of fasting blood glucose, insulin, total cholesterol, triglycerides were measured. Dysfunction of the pancreatic β-cell were observed by immunohistochemistry. The aortic fatty streak-lesions were quantified following lipid staining with Sudan IV. Lipid droplets in liver were observed by Oil red O staining. Results During the feeding period, HFSD induced hyperglycemia, hypercholesterolemia and hypertriglyceridemia. At the end of 8 months, in HFSD-fed animals, the adipocytes were hypertrophic. Injured pancreatic β-cells were observed. The aortic fatty streak-lesions were clearly presented in the animals' aortas. Extensive fat deposited in the liver were observed. Conclusion Our study successfully established a new animal model that feeding minipigs with high fat/high sucrose diet without adding dietary cholesterol can induce insulin resistance, mild diabetes, and atherosclerotic lesions. High fat/high sucrose diet-fed minipigs may be a good animal model for research on the treatment of diabetic dyslipidemia complicated with atherosclerosis.

关 键 词：病理学与病理生理学 饮食诱导的糖尿病伴动脉粥样硬化模型 动物实验 贵州小香猪 致糖尿病饲料 糖尿病 动脉粥样硬化 

分 类 号：R363[医药卫生—病理学]