大鼠种植型肝癌无水乙醇瘤内注射的实验研究  

Experimental study of percutaneous ethanol injection (PEI) on rats with implanted liver tumor

作　　者：邵成伟[1] 王培军[1] 田建明[1] 张火俊[1] 左长京[1] 郝西彦[1] 任方远[1] 袁庆中[1] 李树平[1] 

机构地区：[1]第二军医大学长海医院放射科,上海200433

出　　处：《放射学实践》2003年第6期387-389,共3页Radiologic Practice

基　　金：国家自然科学基金资助课题 (30 0 70 2 33)

摘　　要：目的 :探讨无水乙醇瘤内注射 (PEI)疗法对大鼠种植型肝癌的治疗效果。方法 :制作大鼠肝癌动物模型 ,应用PEI疗法进行治疗 ,1周后用影像学和病理检查观察肿瘤大小、坏死、凋亡变化 ,与对照组比较 ,并观察生存期的长短。结果 :PEI治疗前 ,大鼠肝肿瘤平均体积为 ( 10 0± 6)mm3 ,乙醇治疗后 1周 ,大鼠肝肿瘤平均体积为 ( 12 0± 10 )mm3 ,而荷瘤对照组肝肿瘤平均体积为 ( 190± 11)mm3 ;治疗组肿瘤生长率为 2 0 % ,而荷瘤对照组肝肿瘤生长率为 90 % (P <0 .0 5 )。治疗组病理切片光镜下肿瘤明显凝固性坏死 ,并可诱发细胞凋亡 ;生存期明显延长。结论 :PEI可明显抑制肿瘤生长 ,促进肿瘤凝固性坏死 ,诱发细胞凋亡 。Objective:To study the effects of percutaneous ethanol injection (PEI) on rats with implanted liver tumor.Methods:Direct cancerous ascites injection method was used to make rats liver tumor model,then the liver tumor were treated with PEI.Tumor size,necrosis and apoptosis were observed after one week by imaging and pathology method.The results were compared with those of control group.The survival times were observed also.Results:Before PEI,rats liver tumor volume were (100±6)mm 3.One week after PEI,tumor volume in PEI group (120±10)mm 3 were significantly smaller than those of control group (190±11)mm 3.Tumor growth rate in PEI group (20%) were obviously lower than those of control group (90%, P <0.05).Coagulation necrosis,apoptosis and survival time in PEI group increased significantly than those of control group.Conclusion:The growth of rats liver tumor is inhibited significantly by PEI,and the survival time will be longer.

关 键 词：大鼠 肝癌 无水乙醇 瘤内注射 细胞凋亡 凝固性坏死 

分 类 号：R735.7[医药卫生—肿瘤] R730.5[医药卫生—临床医学]